Yamaguchi Haruyo, Suzuki Shigekatsu, Osana Yasunori, Kawachi Masanobu
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
Department of Electrical and Electronics Engineering, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 903-0213, Japan.
J Genomics. 2020 Jan 1;8:1-6. doi: 10.7150/jgen.40978. eCollection 2020.
, a bloom-forming cyanobacterium distributed mainly in freshwater environments, can be divided into at least 12 groups (A-K and X) based on multi-locus phylogenetic analyses. In this study, we characterized the genome of microcystin-producing NIES-102, assigned to group A, isolated from Lake Kasumigaura, Japan. The complete genome sequence of NIES-102 comprised a 5.87-Mbp circular chromosome containing 5,330 coding sequences. The genome was the largest among all sequenced genomes for the species. In a comparison with the genome of NIES-843, which belongs to the same group, the microcystin biosynthetic gene cluster and CRISPR-Cas locus were highly similar. A synteny analysis revealed small-scale rearrangements between the two genomes. Genes encoding transposases were more abundant in these two genomes than in other genomes. Our results improve our understanding of structural genomic changes and adaptation to a changing environment in the species.
作为一种主要分布在淡水环境中的形成水华的蓝藻细菌，基于多位点系统发育分析，可至少分为12个组（A - K和X）。在本研究中，我们对产自日本霞浦湖、归为A组的产微囊藻毒素的NIES - 102的基因组进行了特征分析。NIES - 102的完整基因组序列由一个5.87 Mbp的环状染色体组成，包含5330个编码序列。该基因组是该物种所有已测序基因组中最大的。与同一组的NIES - 843的基因组相比，微囊藻毒素生物合成基因簇和CRISPR - Cas位点高度相似。共线性分析揭示了这两个基因组之间的小规模重排。这两个基因组中编码转座酶的基因比其他基因组中的更丰富。我们的结果增进了我们对该物种结构基因组变化以及对不断变化环境的适应性的理解。
