Peres Sabine, Fromion Vincent
LRI, Université Paris-Sud, CNRS, Université Paris-Saclay, 91405, Orsay, France.
INRA, UR1404, MaIAGE, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Methods Mol Biol. 2020;2088:359-367. doi: 10.1007/978-1-0716-0159-4_17.
Networks of reactions inside the cell are constrained by the laws of mass and energy balance. Constrained-based modelling (CBM) is the most used method to describe the mass balance of metabolic network. The main key concepts in CBM are stoichiometric analysis such as elementary flux mode analysis or flux balance analysis. Some of these methods have focused on adding thermodynamics constraints to eliminate non-physical fluxes or inconsistencies in the metabolic system. Here, we review the main different approaches and how they tackle the different class of problems.
细胞内的反应网络受到质量和能量平衡定律的限制。基于约束的建模（CBM）是描述代谢网络质量平衡最常用的方法。CBM中的主要关键概念是化学计量分析，如基本通量模式分析或通量平衡分析。其中一些方法专注于添加热力学约束，以消除代谢系统中的非物理通量或不一致性。在这里，我们回顾了主要的不同方法以及它们如何解决不同类型的问题。
