Hanif Muhammad, Mehmood Malik Hassan, Ishrat Ghazala, Abdullah Ahmed, Sohail Samar, Ahmed Mansoor, Gilani Anwarul-Hassan
Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Sindh, Pakistan/Department of Pharmaceutical Chemistry & Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Sindh, Pakistan/Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan.
Pak J Pharm Sci. 2019 Sep;32(5(Special)):2527-2533.
Hippophae rhamnoides (Family; Elaeagnaceae) fruit extract was investigated for prokinetic and gut excitatory effects to rationalize its therapeutic utility in gastrointestinal complaints like delayed gastric emptying and constipation. The fruit extract of Hippophae rhamnoides (Hr.Cr) prepared in hydro-methanol (30:70) was verified for flavonoids, tannins, coumarins and terpenes as plant constituents. In mice, Hr.Cr administration caused an increased in faecal production and charcoal meal transport (50-300mg/kg, per-oral.), similar to activity pattern of carbamylcholine (1 mg/kg). Laxative and prokinetic effects of Hr.Cr were found partially atropine-sensitive. On challenge with isolated intestinal tissues, Hr.Cr charged a dose-dependent spasmogenic effect on jejunum (0.01-1mg/mL) preparations of rabbit and in ileal tissues (guinea-pig) at the dose range of 0.03 to 3mg/mL, following predominant relaxing impact at increased concentrations. Unlike carbamylcholine, stimulant effect of Hr.Cr was partly antagonized in atropine incubated tissues. These data attest the laxative, prokinetic and gut excitatory activities of Hippophae rhamnoides probably mediated through partial activation of muscarinic receptors. Further in agreement of the current findings with earlier reports on gastric emptying effects of Hippophae rhamnoides seed oil, this is the first study of its kind providing insight into mechanism to the laxative potential of Hippophae rhamnoides fruit, thus rationalizing its medicinal use in constipation.
对沙棘（胡颓子科）果实提取物的促动力和肠道兴奋作用进行了研究，以阐明其在治疗诸如胃排空延迟和便秘等胃肠道疾病中的治疗效用。用氢甲醇（30:70）制备的沙棘果实提取物（Hr.Cr），对其中的黄酮类、单宁类、香豆素类和萜类等植物成分进行了验证。在小鼠中，口服给予Hr.Cr（50 - 300mg/kg）可使粪便产生量增加，并加快炭末推进率，其作用模式与氨甲酰胆碱（1mg/kg）相似。发现Hr.Cr的缓泻和促动力作用部分对阿托品敏感。在用离体肠组织进行试验时，Hr.Cr对兔空肠制剂（0.01 - 1mg/mL）以及豚鼠回肠组织（0.03至3mg/mL剂量范围）呈现剂量依赖性的致痉挛作用，而在较高浓度时则主要表现为松弛作用。与氨甲酰胆碱不同，Hr.Cr的刺激作用在经阿托品孵育的组织中部分被拮抗。这些数据证明沙棘的缓泻、促动力和肠道兴奋活性可能是通过部分激活毒蕈碱受体介导的。进一步结合当前研究结果与早期关于沙棘籽油对胃排空作用的报道，这是同类研究中首次深入探究沙棘果实缓泻潜力机制的研究，从而阐明了其在便秘治疗中的药用原理。
