Department of Pharmacy, University of Peshawar, Peshawar, 25120, Pakistan.
BMC Complement Altern Med. 2013 Mar 27;13:70. doi: 10.1186/1472-6882-13-70.
The present study was aimed to provide ethnopharmacological basis for the medicinal use of Viola betonicifolia whole plant in indigestion and constipation.
Mice were used in in-vivo prokinetic and laxative studies while in-vitro experiments were conducted on isolated tissues of rabbit and guinea-pig gut preparations suspended in a tissue bath to measure isotonic contractions.
The crude methanolic extract of Viola betonicifolia (VBME) showed partially atropine-sensitive prokinetic (50 and 100 mg/kg) and laxative (30 and 100 mg/kg) activities in mice. When tested in isolated rabbit jejunum and guinea-pig ileum, VBME caused dose-dependent contractions at 0.01-0.3 mg/mL and 0.03-5 mg/mL, respectively. The spasmogenic effect was partially sensitive to atropine, while the presence of pyrilamine, SB203186 or hexamethonium had no effect in both gut preparations. VBME partially inhibited acetylcholinesterase enzyme (19%) in the in-vitro assay. The spasmodic effect of VBME was more efficacious in guinea-pig ileum than rabbit jejunum preparation. The phytochemical analysis of the crude methanolic extract for total alkaloids and saponins revealed that the VBME is a rich source of alkaloids and saponins.
This study showed the prokinetic and laxative effects of Viola betonicifolia in mice, partially mediated through cholinergic action. The in-vitro spasmodic effect of the plant extract was also partially sensitive to atropine indicating more than one mechanisms in the gut stimulant effect. This study provides a rationale for the medicinal use of Viola betonicifolia in indigestion and constipation.
本研究旨在为 Viola betonicifolia 全草在消化不良和便秘中的药用提供民族药理学基础。
在体内促动力和通便研究中使用小鼠，而在离体组织实验中，将兔和豚鼠肠制备物悬挂在组织浴槽中，测量等张收缩，使用兔离体空肠和豚鼠回肠进行离体实验。
Viola betonicifolia（VBME）的粗甲醇提取物在小鼠中显示部分阿托品敏感的促动力（50 和 100mg/kg）和通便（30 和 100mg/kg）活性。当在兔离体空肠和豚鼠回肠中测试时，VBME 在 0.01-0.3mg/mL 和 0.03-5mg/mL 时分别引起剂量依赖性收缩。痉挛效应部分对阿托品敏感，而在两种肠制备物中，吡咯拉明、SB203186 或六烃季铵无作用。VBME 部分抑制体外测定中的乙酰胆碱酯酶（19%）。VBME 在豚鼠回肠中的痉挛作用比兔空肠制剂更有效。粗甲醇提取物的总生物碱和皂苷的植物化学分析表明，VBME 是生物碱和皂苷的丰富来源。
本研究表明 Viola betonicifolia 在小鼠中的促动力和通便作用，部分通过胆碱能作用介导。植物提取物的体外痉挛作用也部分对阿托品敏感，表明在肠道刺激作用中有多种机制。这项研究为 Viola betonicifolia 在消化不良和便秘中的药用用途提供了依据。
