Hanif Muhammad, Mehmood Malik Hassan, Ishrat Ghazala, Virji Safna Naozer, Malik Abdul, Ahmed Mansoor, Gilani Anwarul-Hassan
Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Sindh, Pakistan/Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Sindh, Pakistan/Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan.
Pak J Pharm Sci. 2019 Sep;32(5(Supplementary)):2347-2355.
Alcea rosea L. also known as Althea rosea belongs to the Malvaceae family. This medicinal herb, traditionally used to treat several conditions including airway disorders like asthma and chronic bronchitis. This study evaluated the bronchodilatory effects and possible mechanism of A. rosea on guinea-pig tracheal tissues. Moreover lipophilic profiling of A. rosea has been carried out by using Gas-Chromatography-Mass-Spectrometry. A total of 19 compounds have been identified from the plant, n-hexane fraction. These compounds have been further confirmed from their Van den Dool and Kratz (I) Indices. Major class of metabolite identified from the plant includes fatty acid, saturated and unsaturated fatty acid esters. Hydrocarbons have also been detected from the n-hexane fraction. These fatty acid esters have not been reported previously by GC-MS and were identified first time from the flowers of Alcea rosea. In-vitro experiments were performed on guinea-pig tracheal tissues, mounted in Kreb's solution at 37°C and bubbled with carbogen. In isolated guinea-pig trachea, A. rosea inhibited carbamylcholine and K+ (80 mM)-induced contractions, potentiated isoprenaline concentration-response curves (CRCs) and suppressed Ca CRCs. These results suggest that A. rosea cause bronchodilation through dual inhibition of phosphodiesterase enzyme and Ca influx, which substantiate its potential in airways disorders.
蜀葵，也被称为药葵，属于锦葵科。这种草药传统上用于治疗多种病症，包括哮喘和慢性支气管炎等气道疾病。本研究评估了蜀葵对豚鼠气管组织的支气管扩张作用及其可能的机制。此外，还通过气相色谱 - 质谱联用技术对蜀葵进行了亲脂性分析。从该植物的正己烷馏分中总共鉴定出19种化合物。这些化合物通过其范登杜尔和克拉茨（I）指数得到进一步确认。从该植物中鉴定出的主要代谢物类别包括脂肪酸、饱和和不饱和脂肪酸酯。在正己烷馏分中也检测到了烃类。这些脂肪酸酯此前尚未通过气相色谱 - 质谱联用技术报道过，首次从蜀葵的花朵中鉴定出来。在体外实验中，对置于37°C的克雷布斯溶液中并用混合气体鼓泡的豚鼠气管组织进行了实验。在离体豚鼠气管中，蜀葵抑制了氨甲酰胆碱和K +（80 mM）诱导的收缩，增强了异丙肾上腺素浓度 - 反应曲线（CRC）并抑制了钙CRC。这些结果表明，蜀葵通过双重抑制磷酸二酯酶和钙内流导致支气管扩张，这证实了其在气道疾病方面的潜力。
