Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University.
Department of Health and Nutrition, Faculty of Health and Nutrition, Osaka Shoin Women's University.
Planta Med. 2020 Feb;86(3):198-204. doi: 10.1055/a-1078-7860. Epub 2020 Jan 2.
The physiological functions of the leaves of Japanese big-leaf magnolia () against enterotoxins produced by foodborne pathogens remain unclear. In this study, we evaluated the protective effects of leaf extract (MLE) against the cytotoxicity of enterotoxin (CPE), which causes the symptoms of type A food poisoning. The protective effects of MLE against CPE-induced cytotoxicity were evaluated in human intestinal epithelial Caco-2 cells. Pre-treatment with MLE significantly suppressed the cytotoxicity induced by CPE in undifferentiated and differentiated human intestinal Caco-2 cells at a pH range of 4.0 - 7.0. This CPE-suppressive effect was due to a hydrophilic sugar-containing compound without phenolic and protein structures but not the hydrophobic biologically active neolignans, honokiol and magnolol. MLE had a protective effect against cytotoxicity caused by type A . Our results provide novel insight regarding the usage of in managing food poisoning.
日本厚朴叶对食源性病原体产生的肠毒素的生理功能尚不清楚。本研究评估了厚朴叶提取物（MLE）对细胞毒素（CPE）细胞毒性的保护作用，CPE 可引起 A 型食物中毒的症状。在未分化和分化的人肠道 Caco-2 细胞中评估 MLE 对 CPE 诱导的细胞毒性的保护作用。MLE 的预处理可显著抑制 pH 值为 4.0-7.0 时未分化和分化的人肠道 Caco-2 细胞中 CPE 诱导的细胞毒性。这种抑制 CPE 的作用是由于含有亲水糖的化合物，而不是具有酚和蛋白质结构的疏水性生物活性新木脂素，厚朴酚和厚朴酚。MLE 对 A 型细胞毒素具有保护作用。我们的研究结果为厚朴在管理食物中毒方面的应用提供了新的见解。
