Lovecká Petra, Svobodová Alžběta, Macůrková Anna, Vrchotová Blanka, Demnerová Kateřina, Wimmer Zdeněk
Department of Biochemistry and Microbiology, University of Chemistry and Technology in Prague, Technická 3, 166 28 Prague 6, Czech Republic.
Department of Chemistry of Natural Compounds, University of Chemistry and Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Plants (Basel). 2020 Jul 11;9(7):879. doi: 10.3390/plants9070879.
plants are used both as food supplements and as cosmetic and medicinal products. The objectives of this work consisted of preparing extracts from leaves and flowers of eight plants, and of determining concentrations of magnolol (1 to 100 mg·g), honokiol (0.11 to 250 mg·g), and obovatol (0.09 to 650 mg·g), typical neolignans for the genus , in extracts made by using a methanol/water (80/20) mixture. The tested plants, over sixty years old, were obtained from Průhonický Park (Prague area, Czech Republic): MTR 1531, MOB 1511, and six hybrid plants , results of a crossbreeding of MTR 1531 with MOB 1511. The identification of neolignans was performed by HRMS after a reversed-phase high-performance liquid chromatography (RP-HPLC) fractionation of an extract from MTR 1531. The highest concentrations of neolignans were found in the flowers, most often in their reproductive parts, and obovatol was the most abundant in every tested plant. The highest concentrations of neolignans were detected in parent plants, and lower concentrations in hybrid magnolias. Flower extracts from the parent plants MTR 1531 and MOB 1511, flower extracts from the hybrid plants MPR 0271, MPR 0151, and MPR 1531, and leaf extract from the hybrid plant MPR 0271 inhibited growth of .
植物既被用作食品补充剂,也被用作化妆品和药品。这项工作的目标包括从八种植物的叶子和花朵中制备提取物,并测定在使用甲醇/水(80/20)混合物制成的提取物中厚朴酚(1至100毫克·克)、和厚朴酚(0.11至250毫克·克)以及鹅掌楸醇(0.09至650毫克·克)的浓度,这些是该属典型的新木脂素。所测试的这些六十多年树龄的植物取自普鲁霍尼采公园(捷克共和国布拉格地区):MTR 1531、MOB 1511以及六种杂交植物,即MTR 1531与MOB 1511杂交的结果。新木脂素的鉴定是在对MTR 1531的提取物进行反相高效液相色谱(RP - HPLC)分离后通过高分辨质谱法进行的。在花朵中发现新木脂素的浓度最高,最常见于其生殖部位,并且鹅掌楸醇在每种测试植物中含量最为丰富。在亲本植物中检测到新木脂素的最高浓度,而在杂交木兰中浓度较低。亲本植物MTR 1531和MOB 1511的花提取物、杂交植物MPR 0271、MPR 0151和MPR 1531的花提取物以及杂交植物MPR 0271的叶提取物均抑制了……的生长。