c-Myc-Induced Long Non-Coding RNA Small Nucleolar RNA Host Gene 7 Regulates Glycolysis in Breast Cancer.

PURPOSE

Recent studies have shown that long non-coding RNA (lncRNA) play an important role in cancer metabolism and development. The lncRNA small nucleolar RNA host gene 7 () was reported to be upregulated in colorectal cancer and contribute to its progression. In the current study, we investigated the role of lncRNA- in breast cancer and explored the underlying mechanism.

METHODS

We monitored the expression of lncRNA- in breast cancer tissues and breast cancer cell lines. We evaluated the effects of lncRNA- on cell proliferation and glycolysis in breast cancer cells by knocking down or overexpressing lncRNA-. We searched for the potential microRNA (miRNA) target of lncRNA- and evaluated the effects of the target miRNA on glycolysis. We evaluated the potential regulation of lncRNA- by c-Myc.

RESULTS

LncRNA- was up-regulated in both breast cancer tissues and breast cancer cell lines. Knocking down lncRNA- inhibited breast cancer cell proliferation while overexpressing lncRNA- enhanced cell proliferation. Knocking down lncRNA- resulted in decreased expression of lactate dehydrogenase A (LDHA) and decreased glycolysis. LncRNA- targeted miR-34a-5p to regulate LDHA expression and glycolysis. c-Myc bound to promoter of lncRNA- and positively regulated lncRNA- expression.

CONCLUSION

We demonstrated that c-Myc regulated glycolysis through the lncRNA-/miR-34a-5p/LDHA axis in breast cancer cells.