College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong, 273165, People's Republic of China.
Institute of Functional Materials and Agricultural Applied Chemistry, College of Science, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
Anal Bioanal Chem. 2020 Feb;412(4):841-848. doi: 10.1007/s00216-019-02283-2. Epub 2020 Jan 2.
A sensitive photoelectrochemical (PEC) aptasensor was constructed for prostate-specific antigen (PSA) detection using an enhanced photocurrent response strategy. The p-n heterostructure CdS-CuO nanorod arrays were prepared on Ti mesh (CdS-CuO NAs/TM) by a simple hydrothermal method and successive ionic-layer adsorption reactions. Compared with the original CdS/TM, the synergistic effect of p-n type CdS-CuO NAs/TM and the internal electric field realizes the effective separation of photoinduced electron-hole pairs and improves the PEC performance. In order to construct the aptasensor, an amino-modified aptamer was immobilized on CdS-CuO NAs/TM to serve as a recognition unit for PSA. After the introduction of PSA, PSA was specifically captured by the aptamer on the PEC aptasensor, which can be oxidized by photogenerated holes to prevent electron-hole recombination and increase photocurrent. Under optimal conditions, the constructed PEC aptasensor has a linear range of 0.1-100 ng·mL and a detection limit as low as 0.026 ng·mL. The results of aptasensor detection of human serum indicate that it has broad application prospects in biosensors and photoelectrochemical analysis.
一种基于增强光电化学（PEC）响应策略的用于前列腺特异性抗原（PSA）检测的灵敏 PEC 适体传感器被构建出来。通过简单的水热法和连续离子层吸附反应，在钛网（CdS-CuO NAs/TM）上制备了 p-n 异质结构的 CdS-CuO 纳米棒阵列。与原始的 CdS/TM 相比，p-n 型 CdS-CuO NAs/TM 的协同效应和内电场实现了光生电子-空穴对的有效分离，提高了 PEC 性能。为了构建适体传感器，将氨基修饰的适体固定在 CdS-CuO NAs/TM 上作为 PSA 的识别单元。引入 PSA 后，PSA 被 PEC 适体传感器上的适体特异性捕获，可被光生空穴氧化，防止电子-空穴复合，增加光电流。在最佳条件下，所构建的 PEC 适体传感器的线性范围为 0.1-100 ng·mL，检测限低至 0.026 ng·mL。适体传感器对人血清的检测结果表明，它在生物传感器和光电化学分析中有广阔的应用前景。
