Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Epitranscriptomics and RNA Dynamics, Centre for Genomic Regulation, Barcelona, Spain.
Parasitol Res. 2020 Feb;119(2):749-753. doi: 10.1007/s00436-019-06554-w. Epub 2020 Jan 3.
Trypanosoma cruzi, the causative agent of Chagas disease, has a complex life cycle that requires the adaptation to different environments. In the absence of traditional mechanisms for regulation of gene expression, this parasite relies on posttranscriptional control events, which allow the progression of its life cycle in different hosts and stress conditions. In this context, different stress conditions trigger the aggregation of RNA-binding proteins and their target mRNAs into cytoplasmic foci known as RNA granules, which act as RNA-sorting centers. In this study, we have characterized the T. cruzi RNA-binding protein ALBA30 during nutritional stress conditions. Using a recombinant form of TcALBA30 to facilitate its detection (rTcALBA30), we showed that this protein resides in the cytoplasm in normal growth conditions but is recruited into cytoplasmic foci after starvation. Moreover, evaluation of rTcALBA30 in parasites that reached the stationary phase of growth also showed the recruitment of this protein into cytoplasmic foci. Our results indicate that, similar to TbALBA3, TcALBA30 aggregates into stress granules in parasites submitted to nutritional stress.
克氏锥虫，恰加斯病的病原体，具有复杂的生命周期，需要适应不同的环境。在缺乏传统的基因表达调控机制的情况下，这种寄生虫依赖于转录后控制事件，这使得它能够在不同的宿主和应激条件下完成生命周期。在这种情况下，不同的应激条件会触发 RNA 结合蛋白及其靶 mRNA 聚集到细胞质焦点，即 RNA 颗粒，这些颗粒充当 RNA 分拣中心。在这项研究中，我们对克氏锥虫 RNA 结合蛋白 ALBA30 在营养应激条件下进行了表征。我们使用重组形式的 TcALBA30 来促进其检测(rTcALBA30)，结果表明，在正常生长条件下，该蛋白位于细胞质中，但在饥饿后会被招募到细胞质焦点中。此外，对处于生长静止期的寄生虫进行 rTcALBA30 的评估也显示了该蛋白被招募到细胞质焦点中。我们的结果表明，与 TbALBA3 类似，TcALBA30 在受到营养应激的寄生虫中聚集到应激颗粒中。
