Zhang Jingjie, Song Pengfei, Jiang Feng, Zhang Tongzuo
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
Front Microbiol. 2024 Aug 21;15:1439554. doi: 10.3389/fmicb.2024.1439554. eCollection 2024.
The differences in gut microbiota among different populations, to a certain extent, reflect the degree of interaction between individuals within populations. To assess the interaction levels among several small populations of Przewalski's gazelle () ( = 105, from seven different regions) based on differences in gut microbiota, we used the closely related Tibetan gazelle () ( = 52, from seven different regions) as a control. We then compared the gut microbial communities between different populations of the two species using high-throughput sequencing of the 16S rRNA gene. The results showed that within a 100 km geographical distance, the intergroup differences in relative abundance of dominant bacteria, α-diversity, β-diversity, and functional metabolism abundance were higher or significantly higher in Przewalski's gazelle (narrowly distributed species) compared to the Tibetan gazelle (widely distributed species). Additionally, the proportion of shared OTUs between groups in Przewalski's gazelle was significantly lower than in Tibetan gazelle ( < 0.05). Additionally, neutral community model results also showed lower dispersal limitation in the Tibetan gazelle compared to Przewalski's gazelle. Therefore, based on the above results, we comprehensively speculate that the spatial interaction degree of Przewalski's gazelle in different habitat patches is relatively low. This study, starting from the perspective of gut microbiota, adopts a non-genetic perspective or method to assess whether there is, or to what extent there is, close interaction between species populations.
不同种群之间肠道微生物群的差异在一定程度上反映了种群内个体间的相互作用程度。为了基于肠道微生物群的差异评估普氏原羚（ ）几个小种群（ = 105，来自七个不同地区）之间的相互作用水平，我们使用了亲缘关系较近的藏羚（ ）（ = 52，来自七个不同地区）作为对照。然后，我们使用16S rRNA基因的高通量测序比较了这两个物种不同种群之间的肠道微生物群落。结果表明，在100公里的地理距离内，与藏羚（广泛分布物种）相比，普氏原羚（狭域分布物种）中优势细菌相对丰度、α多样性、β多样性和功能代谢丰度的组间差异更高或显著更高。此外，普氏原羚组间共享OTU的比例显著低于藏羚（ < 0.05）。此外，中性群落模型结果也表明，与普氏原羚相比，藏羚的扩散限制更低。因此，基于上述结果，我们综合推测普氏原羚在不同栖息地斑块中的空间相互作用程度相对较低。本研究从肠道微生物群的角度出发，采用非遗传视角或方法来评估物种种群之间是否存在密切相互作用，或在何种程度上存在密切相互作用。
