Fredricsson B, Waxegård G, Brege S, Lundberg I
Fertil Steril. 1977 Feb;28(2):168-74. doi: 10.1016/s0015-0282(16)42377-0.
Live and dead spermatozoa of human semen were distinguished and evaluated separately with respect to their morphology after supravital staining with buffered eosin. This evaluation was shown to be almost as reliable as that of the commonly stained smear. Different samples from the same individual were found to be similar with respect to both the total population and the live population. Different types of abnormalities were not equally common in the live and dead populations. Tapered heads were more common in the live population and abnormal midpieces more common in the dead, whereas abnormal tails and cytoplasmic droplets occurred with the same frequency in both populations. However, neither the frequency of all spermatozoal abnormalities nor that of abnormal heads or midpieces studied separately showed strong correlations in the two populations, in contrast to the frequencies of tapered heads, cytoplasmic droplets, and abnormal tails. No constant relationship was found between the live population and the entire sperm population with respect to numerical differences or quotients between the figures for abnormal cell frequency.Therefore, study of the morphology of the live sperm population gives supplementary information about the qualities of semen not attained by previous methods of study.
在用缓冲伊红进行超活染色后，分别对人类精液中的活精子和死精子的形态进行了区分和评估。结果表明，这种评估几乎与常规染色涂片的评估一样可靠。发现同一个体的不同样本在总精子数和活精子数方面都相似。不同类型的异常在活精子和死精子群体中的常见程度并不相同。锥形头在活精子群体中更常见，异常中段在死精子中更常见，而异常尾部和细胞质滴在两个群体中的出现频率相同。然而，与锥形头、细胞质滴和异常尾部的频率不同，无论是所有精子异常的频率，还是单独研究的异常头部或中段的频率，在这两个群体中都没有显示出很强的相关性。在活精子群体和整个精子群体之间，就异常细胞频率数字之间的差异或商而言，未发现恒定关系。因此，对活精子群体形态的研究提供了有关精液质量的补充信息，这是以前的研究方法所无法获得的。
