Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, P.R. China.
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
J Invest Surg. 2021 Aug;34(8):853-861. doi: 10.1080/08941939.2019.1705443. Epub 2020 Jan 6.
Gastric cancer is the third leading cause of cancer-related mortality worldwide. Most tripartite motif (TRIM) family proteins are known as E3 ubiquitin ligases and considerable previous research has revealed the involvement of TRIM proteins in carcinogenesis. TRIM15 is a protein from the TRIM family and the aim of this study is to investigate the role of TRIM15 in gastric cancer.
We conducted immunohistochemical staining to examine TRIM15 expression using samples from Zhongshan Hospital of Fudan University. We also conducted transwell assay as well as western blot by using gastric cancer cells.
The expression of TRIM15 in gastric cancer tissues was higher than normal tissues. Present data demonstrated that high TRIM15 staining intensity had a positive relation to tumor invasion depth ( = 0.007), lymph node metastasis ( = 0.013), distant metastasis ( = 0.031), the tumor-node-metastasis (TNM) staging system ( = 0.002) and shorter overall survival (OS) in gastric cancer patients ( < 0.001). It was also worthwhile mentioning that TRIM15 was an adverse prognostic variable for OS. To gain more insight, we incorporated TRIM15 expression into the tumor-node-metastasis (TNM) staging system and thus established a nomogram. Data derived from the nomogram suggested that fitting TRIM15 expression into the prognostic model exhibited better efficiency for predicting OS in gastric cancer patients. Furthermore, TRIM15 promoted migration, invasion and epithelial-mesenchymal transition of gastric cancer cells.
Together, TRIM15 expression was found as a specific and independent adverse predictor in gastric cancer patients and the nomogram may contribute to better clinical management.
胃癌是全球导致癌症相关死亡的第三大主要原因。大多数三联基序（TRIM）家族蛋白被认为是 E3 泛素连接酶，大量先前的研究表明 TRIM 蛋白参与了癌症的发生。TRIM15 是 TRIM 家族的一种蛋白质，本研究旨在探讨 TRIM15 在胃癌中的作用。
我们使用来自复旦大学中山医院的样本进行免疫组织化学染色，以检查 TRIM15 的表达。我们还使用胃癌细胞进行了 Transwell 测定和 Western blot。
胃癌组织中 TRIM15 的表达高于正常组织。目前的数据表明，高 TRIM15 染色强度与肿瘤浸润深度（= 0.007）、淋巴结转移（= 0.013）、远处转移（= 0.031）、肿瘤-淋巴结-转移（TNM）分期系统（= 0.002）和胃癌患者的总生存期（OS）较短（<0.001）有关。值得一提的是，TRIM15 是 OS 的不良预后变量。为了更深入地了解这一点，我们将 TRIM15 表达纳入 TNM 分期系统，并建立了一个列线图。来自列线图的数据表明，将 TRIM15 表达纳入预后模型可以更好地预测胃癌患者的 OS。此外，TRIM15 促进了胃癌细胞的迁移、侵袭和上皮-间充质转化。
TRIM15 表达被发现是胃癌患者的特异性和独立的不良预测因子，该列线图可能有助于更好的临床管理。
