溶酶体相关膜蛋白 1（LAMP1）在哺乳动物细胞系中的不同定位。
Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.
机构信息
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Department of System Chemotherapy and Molecular Sciences, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
出版信息
Histochem Cell Biol. 2020 Apr;153(4):199-213. doi: 10.1007/s00418-019-01842-z. Epub 2020 Jan 6.
Lysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines. Rat LAMP1 fused to enhanced green fluorescent protein (EGFP) mostly accumulated at a particular cytoplasmic area and barely co-localized with LysoTracker® Red DND-99 in golden hamster kidney BHK-21 cells and Chinese hamster ovary CHO-K1 cells. Golden hamster, Chinese hamster, and human LAMP1-EGFP showed a similar intracellular distribution to rat LAMP1-EGFP in BHK-21 cells. Endogenous LAMP1 was also detected in a perinuclear area in BHK-21 cells and CHO-K1 cells, and co-localized with rat CD63-EGFP in BHK-21 cells. Moreover, rat LAMP1-DsRed-Monomer co-localized well with the human trans-Golgi network protein 2-EGFP in BHK-21 cells. These results reveal that LAMP1 predominantly localizes to the trans-Golgi network in BHK-21 cells.
溶酶体相关膜蛋白 1（LAMP1）主要定位于溶酶体和晚期内体。在此，我们研究了五种哺乳动物培养细胞系中溶酶体膜蛋白的细胞内定位。与增强型绿色荧光蛋白（EGFP）融合的大鼠 LAMP1 主要积聚在特定的细胞质区域，几乎不在金黄仓鼠肾 BHK-21 细胞和中国仓鼠卵巢 CHO-K1 细胞中与 LysoTracker® Red DND-99 共定位。在 BHK-21 细胞中，金黄仓鼠、中国仓鼠和人 LAMP1-EGFP 与大鼠 LAMP1-EGFP 表现出相似的细胞内分布。内源性 LAMP1 在 BHK-21 细胞和 CHO-K1 细胞中也检测到在核周区，并与 BHK-21 细胞中的大鼠 CD63-EGFP 共定位。此外，大鼠 LAMP1-DsRed-Monomer 与 BHK-21 细胞中的人高尔基网络蛋白 2-EGFP 很好地共定位。这些结果表明 LAMP1 主要定位于 BHK-21 细胞中的高尔基网络。
Zotero 插件