Breimer M E, Karlsson H, Karlsson K A, Nilson K, Samuelsson B E, Strömberg N
Department of Medical Biochemistry, University of Göteborg, Sweden.
Carbohydr Res. 1988 Jul 15;178:111-20. doi: 10.1016/0008-6215(88)80105-8.
Two glycolipid fractions, isolated in 1975 from blood group A1 erythrocytes and shown on the basis of direct-inlet mass spectrometry to contain eight- and nine-sugar A-type sequences, have been reinvestigated by fast-atom-bombardment mass spectrometry and overlay analysis with selected monoclonal anti-A antibodies. The presence of three separate glycolipids was concluded, consistent with a common paragloboside backbone [beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-D-Glc] and a typical erythrocyte ceramide component (sphingosine, and 22-, 23-, 24-, and 25-carbon nonhydroxy fatty acids). It is proposed that they carry A determinants based on Type 1 [beta-D-Galp-(1----3)-beta-D-GlcpNAc], Type 2 [beta-D-Galp-(1----4)-beta-D-GlcpNAc], and Type 3 [beta-D-Galp-(1----3)-alpha-D-GalpNAc] chains, respectively. The Type 1 (eight sugars) and Type 3 (nine sugars) glycolipids appeared in mixtures of both the native and the acetylated form. The existence of Type 1 glycolipid, which appears to be a genuine erythrocyte glycolipid as concluded from the ceramide composition, had been predicted earlier by other workers.
1975年从A1血型红细胞中分离出两种糖脂组分，通过直接进样质谱分析表明其含有八糖和九糖A型序列。现采用快原子轰击质谱法并结合选定的抗A单克隆抗体进行覆盖分析，对这两种糖脂组分进行了重新研究。得出存在三种不同糖脂的结论，这与常见的副 Globoside 主链[β-D-半乳糖-(1→4)-β-D-葡萄糖胺-(1→3)-β-D-半乳糖-(1→4)-D-葡萄糖]和典型的红细胞神经酰胺成分（鞘氨醇以及22、23、24和25碳的非羟基脂肪酸）一致。有人提出它们分别基于1型[β-D-半乳糖-(1→3)-β-D-葡萄糖胺]、2型[β-D-半乳糖-(1→4)-β-D-葡萄糖胺]和3型[β-D-半乳糖-(1→3)-α-D-半乳糖胺]链携带A决定簇。1型（八糖）和3型（九糖）糖脂以天然形式和乙酰化形式的混合物出现。从神经酰胺组成来看，1型糖脂似乎是一种真正的红细胞糖脂，其他研究人员此前曾预测过它的存在。
