Hanfland P, Kordowicz M, Peter-Katalinić J, Egge H, Dabrowski J, Dabrowski U
Institut für Experimentelle Hämatologie und Bluttransfusionswesen, Universität Bonn, Federal Republic of Germany.
Carbohydr Res. 1988 Jul 15;178:1-21. doi: 10.1016/0008-6215(88)80098-3.
The structures of two glycosphingolipids, a ceramide eicosasaccharide BIrab-3 and a ceramide pentacosasaccharide BIrab-4 with "B-like" and distinct I blood-group activity, isolated in high yield from rabbit erythrocyte membranes, were investigated. The determination of their general structure, alpha-D-Galp-(1----3)-beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----3)- [alpha-D-Galp-(1----3)-beta-D-Galp-(1----4)-beta-D-Glcp-NAc-(1----6)]-be ta- D-Galp-(1----n4)-beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-beta-D-Gl cp- (1----1)-ceramide, with n = 3 for BIrab-3 and n = 4 for BIrab-4, was based on the results of methylation analysis, fast-atom-bombardment and electron-impact mass spectrometry, 1D and 2D COSY, RCT, and n.O.e. 1H-N.m.r. spectra, and specific enzymic and chemical degradation.
研究了从兔红细胞膜中高产率分离得到的两种糖鞘脂的结构，一种是具有“B样”和独特I血型活性的神经酰胺二十糖BIrab - 3，另一种是神经酰胺二十五糖BIrab - 4。它们的一般结构为α-D-半乳糖-(1→3)-β-D-半乳糖-(1→4)-β-D-葡萄糖胺-(1→3)-[α-D-半乳糖-(1→3)-β-D-半乳糖-(1→4)-β-D-葡萄糖胺-(1→6)]-β-D-半乳糖-(1→n4)-β-D-葡萄糖胺-(1→3)-β-D-半乳糖-(1→4)-β-D-葡萄糖-(1→1)-神经酰胺，其中BIrab - 3的n = 3，BIrab - 4的n = 4，该结构是基于甲基化分析、快原子轰击和电子轰击质谱、一维和二维COSY、RCT以及n.O.e. 1H-N.m.r.光谱结果，以及特定的酶促和化学降解确定的。
