Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Histology Department, Faculty of Medicine, Kafr El Sheikh University, Kafr El Sheikh, Egypt.
Environ Sci Pollut Res Int. 2020 Mar;27(9):9236-9246. doi: 10.1007/s11356-019-06528-6. Epub 2020 Jan 8.
Vitamin B17 (VB17), also known as amygdalin and laetrile, is a type of carbohydrate occurring naturally in many plants, such as apricot kernels which have obtained a great interest in cancer therapy. This study aimed to investigate the hepatic protective potential of VB17 against Ehrlich ascites carcinoma (EAC)-bearing mice-induced liver injury, DNA damage, apoptotic P53, and PCNA alterations. A total of 100 female mice were divided into 5 groups (1st group, control group; 2nd group, VB17 group; 3rd group, EAC group; 4th group, pre-treated EAC with VB17; 5th group, co-treated EAC with VB17). Results showed that the presence of VB17 in pre-treated and co-treated groups lead to decreased DNA damage, microsomal protein, NADPH cytochrome c reductase, alpha-fetoprotein (AFP), AST, ALT, and ALP while showed increased cytochrome b5, cytochrome P450 amidopyrine N-demethylase, and aniline 4-hydroxylase compared with the EAC group. Many histopathological changes were observed in liver sections in EAC as moderate fibrosis and marked diffuse necrosis of hepatic tissue, marked inflammatory cells, and congested blood sinusoids. On the other hand, there was a moderate degree of improvement in hepatocytes in liver sections in pre-treated VB17+EAC, while a mild degree of improvement in hepatocytes, moderate cellular infiltrations, and moderate cytoplasmic vacuolization of hepatocytes in liver sections in co-treated EAC+VB17. In addition, there was a depletion in hepatic P53 and PCNA protein expression compared with the EAC group. It could be concluded that VB17 has a potential hepatoprotective effect against EAC cell-induced liver toxicity.
维生素 B17（VB17），也称为苦杏仁苷和维生素 B17，是一种天然存在于许多植物中的碳水化合物，如杏仁核，在癌症治疗中引起了极大的兴趣。本研究旨在探讨 VB17 对艾氏腹水癌（EAC）荷瘤小鼠诱导的肝损伤、DNA 损伤、凋亡 P53 和 PCNA 改变的肝保护潜力。将 100 只雌性小鼠分为 5 组（第 1 组，对照组；第 2 组，VB17 组；第 3 组，EAC 组；第 4 组，EAC 用 VB17 预处理组；第 5 组，EAC 与 VB17 共同处理组）。结果表明，预处理和共同处理组中 VB17 的存在导致 DNA 损伤、微粒体蛋白、NADPH 细胞色素 c 还原酶、甲胎蛋白（AFP）、AST、ALT 和 ALP 减少，而细胞色素 b5、细胞色素 P450 对乙酰氨基酚 N-脱甲基酶和苯胺 4-羟化酶增加。在 EAC 中观察到肝组织中度纤维化和广泛弥漫性坏死、明显炎症细胞浸润和充血的血窦等许多组织病理学变化。另一方面，在预处理 VB17+EAC 的肝组织切片中，肝细胞有中度改善，而在共同处理 EAC+VB17 的肝组织切片中，肝细胞有轻度改善、中度细胞浸润和肝细胞细胞质空泡化。此外，与 EAC 组相比，肝组织 P53 和 PCNA 蛋白表达减少。可以得出结论，VB17 对 EAC 细胞诱导的肝毒性具有潜在的肝保护作用。
