University of Michigan-Shanghai Jiao Tong University Joint Institute, Shanghai Jiao Tong University, Shanghai, China.
State Key Laboratory of Advanced Optical Communication Systems and Networks, Shanghai Jiao Tong University, Shanghai, China.
J Biophotonics. 2020 Apr;13(4):e201960200. doi: 10.1002/jbio.201960200. Epub 2020 Jan 13.
In this study, a novel photoacoustic microscopy (PAM) probe integrating white-light microscopy (WLM) modality that provides guidance for PAM imaging and complementary information is implemented. One single core of an imaging fiber bundle is employed to deliver a pulsed laser for photoacoustic excitation for PAM mode, which provides high resolution with deep penetration. Meanwhile, for WLM mode, the imaging fiber bundle is used to transmit two-dimensional superficial images. Lateral resolution of 7.2 μm for PAM is achieved. Since miniature components are used, the probe diameter is only 1.7 mm. Imaging of phantom and animals in vivo is conducted to show the imaging capability of the probe. The probe has several advantages by introducing the WLM mode, such as being able to conveniently identify regions of interest and align the focus for PAM mode. The prototype of an endoscope shows potential to facilitate clinical photoacoustic endoscopic applications.
在这项研究中，实现了一种新型的光声显微镜 (PAM) 探头，该探头集成了白光显微镜 (WLM) 模式，为 PAM 成像提供了指导，并提供了补充信息。一个成像光纤束的单个芯用于传输用于 PAM 模式的脉冲激光以进行光声激发，该模式提供了高分辨率和深穿透。同时，对于 WLM 模式，成像光纤束用于传输二维浅层图像。实现了 PAM 的横向分辨率为 7.2μm。由于使用了微型组件，探头直径仅为 1.7mm。对体模和动物进行了体内成像，以显示探头的成像能力。通过引入 WLM 模式，该探头具有几个优点，例如能够方便地识别感兴趣区域并对准 PAM 模式的焦点。内窥镜的原型显示出促进临床光声内窥镜应用的潜力。
