Dai Xianjin, Xi Lei, Duan Can, Yang Hao, Xie Huikai, Jiang Huabei
Opt Lett. 2015 Jun 15;40(12):2921-4. doi: 10.1364/OL.40.002921.
In this Letter, we present a novel tri-modal miniature side-view probe, through which optical-resolution photoacoustic microscopy (OR-PAM), optical coherence tomography (OCT), and pulse-echo ultrasound (US) images can be coaxially acquired and displayed simultaneously. The probe consists of a common optical path for OR-PAM (light delivery) and OCT (light delivery/detection), and a 40-MHz unfocused ultrasound transducer for OR-PAM (photoacoustic detection) and US (ultrasound transmission/receiving) with an overall diameter of 2 mm. Combining OR-PAM, OCT, and US would provide complementary information including optical absorption (OR-PAM), optical back-scattering (OCT), and deep tissue structures (US) about biological tissue. Based on an integrated imaging system consisting of OR-PAM, time-domain OCT, and US, phantom images and in vivo images of rat ear were acquired to demonstrate the capabilities of the integrated tri-modality imaging probe. The probe yields a lateral resolution of 13.6 μm for OR-PAM and OCT, and an axial resolution of 43 μm for OR-PAM and US. Currently, for a scanning area of 1 ×1 mm, it took ∼25 min to acquire data for tri-modal volumetric imaging.
在本信函中，我们展示了一种新型的三模态微型侧视探头，通过该探头可同轴采集并同时显示光学分辨率光声显微镜（OR-PAM）、光学相干断层扫描（OCT）和脉冲回波超声（US）图像。该探头由用于OR-PAM（光传输）和OCT（光传输/检测）的公共光路，以及一个用于OR-PAM（光声检测）和US（超声发射/接收）的40兆赫非聚焦超声换能器组成，其总直径为2毫米。结合OR-PAM、OCT和US能够提供关于生物组织的互补信息，包括光吸收（OR-PAM）、光背散射（OCT）和深部组织结构（US）。基于由OR-PAM、时域OCT和US组成的集成成像系统，采集了大鼠耳朵的体模图像和体内图像以展示集成三模态成像探头的性能。该探头对于OR-PAM和OCT的横向分辨率为13.6微米，对于OR-PAM和US的轴向分辨率为43微米。目前，对于1×1毫米的扫描区域，采集三模态体积成像数据大约需要25分钟。
