Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran.
Department of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran.
Arch Iran Med. 2022 Dec 1;25(12):817-827. doi: 10.34172/aim.2022.127.
Circular RNAs (circRNAs), one of the recent subclasses of non-coding RNAs (ncRNAs), show pivotal functions in regulation of gene expression and have significant roles in malignancies including breast cancer (BC). This study was aimed to assess the hsa_circ_0001445 and hsa_circ_0020397 expression and role in BC, as well as the potential circRNA/miRNA/mRNA crosstalk in these contexts.
The expression of hsa_circ_0001445 and hsa_circ_0020397 in 50 breast tumors and 50 normal tissues adjacent to the tumors was investigated using quantitative real-time polymerase chain reaction (qRT-PCR). Finally, bioinformatics analyses were used to uncover hsa_circ_0001445, hsa_circ_0020397-miRNA-mRNA potential regulatory networks.
The hsa_circ_0001445 expression was considerably downregulated in malignant tissues compared to their normal counterparts (=0.020), while the hsa_circ_0020397 showed an upregulated pattern (<0.001). Additionally, it was observed that the higher expression of hsa_circ_0001445 was associated with hair dye avoidance (=0.034) and normal body mass index (BMI) (=0.016) while hsa_circ_0020397 over-expression had an important association with a lack of vitamin D consumption (=0.039). On the other hand, lower expression of hsa_circ_0001445 was significantly associated with age at menarche ˂14 years (=0.027). Our study also revealed that the two circRNAs have potential ability to regulate key mRNAs and miRNAs in competing endogenous RNA (ceRNA) networks.
It is suggested that hsa_circ_0001445 and hsa_circ_0020397 with two opposite roles may be involved in BC development through sponging some miRNAs regulating ceRNA networks. However, their molecular interactions should be validated by further functional studies.
环状 RNA（circRNA）是最近发现的非编码 RNA（ncRNA）亚类之一，在基因表达调控中具有重要功能，并在包括乳腺癌（BC）在内的恶性肿瘤中发挥重要作用。本研究旨在评估 hsa_circ_0001445 和 hsa_circ_0020397 在 BC 中的表达和作用，以及这些情况下环状 RNA/miRNA/mRNA 相互作用的潜在情况。
使用定量实时聚合酶链反应（qRT-PCR）检测 50 例乳腺癌肿瘤组织和 50 例肿瘤旁正常组织中 hsa_circ_0001445 和 hsa_circ_0020397 的表达。最后，进行生物信息学分析以揭示 hsa_circ_0001445、hsa_circ_0020397-miRNA-mRNA 潜在调控网络。
与相应的正常组织相比，恶性组织中 hsa_circ_0001445 的表达明显下调（=0.020），而 hsa_circ_0020397 呈上调模式（<0.001）。此外，观察到 hsa_circ_0001445 表达较高与避免使用染发剂（=0.034）和正常体重指数（BMI）（=0.016）有关，而 hsa_circ_0020397 过表达与缺乏维生素 D 摄入有重要关联（=0.039）。另一方面，hsa_circ_0001445 表达较低与初潮年龄<14 岁显著相关（=0.027）。我们的研究还表明，这两种 circRNA 具有通过海绵吸附一些 miRNA 调节竞争内源性 RNA（ceRNA）网络来调节关键 mRNAs 和 miRNAs 的潜在能力。然而，它们的分子相互作用应通过进一步的功能研究来验证。
hsa_circ_0001445 和 hsa_circ_0020397 具有两种相反的作用，可能通过海绵吸附一些 miRNA 调节 ceRNA 网络参与乳腺癌的发生发展。然而，它们的分子相互作用需要进一步的功能研究来验证。
