YL-11产生的具有抗肿瘤活性的胞外多糖的遗传和生化特性
Genetic and Biochemical Characterization of an Exopolysaccharide With Antitumoral Activity Produced by YL-11.
作者信息
Wei Yunlu, Li Fei, Li Le, Huang Linlin, Li Quanhong
机构信息
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
National Engineering Research Center for Fruit and Vegetable Processing, Beijing, China.
出版信息
Front Microbiol. 2019 Dec 17;10:2898. doi: 10.3389/fmicb.2019.02898. eCollection 2019.
In the present study, the whole genome sequence of YL-11, a novel exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) strain isolated from fermented milk, was determined. Genetic information and the synthetic mechanism of the EPS in YL-11 were identified based on bioinformatic analysis of the complete genome. The purified EPS of YL-11 mainly comprised galactose (48.0%), glucose (30.3%), mannose (11.8%), and arabinose (6.0%). , the EPS from YL-11 exhibited inhibition activity against HT-29 and Caco-2 colon cancer cells, suggesting that EPS from strain YL-11 might be used as an antitumoral agent. EPS at 600 and 800 μg/mL achieved inhibition rates of 46.5 ± 3.5% and 45.6 ± 6.1% to HT-29 cells, respectively. The genomic information about YL-11 and the antitumoral activity of YL-11 EPS provide a theoretical foundation for the future application of EPS in the food and pharmaceutical industries.
在本研究中，测定了从发酵乳中分离出的一种新型产胞外多糖（EPS）的乳酸菌（LAB）菌株YL-11的全基因组序列。基于对完整基因组的生物信息学分析，确定了YL-11中EPS的遗传信息和合成机制。YL-11纯化后的EPS主要由半乳糖（48.0%）、葡萄糖（30.3%）、甘露糖（11.8%）和阿拉伯糖（6.0%）组成。此外，YL-11产生的EPS对HT-29和Caco-2结肠癌细胞具有抑制活性，这表明YL-11菌株产生的EPS可能用作抗肿瘤剂。EPS浓度为600和800μg/mL时，对HT-29细胞的抑制率分别达到46.5±3.5%和45.6±6.1%。关于YL-11的基因组信息以及YL-11 EPS的抗肿瘤活性为EPS未来在食品和制药行业的应用提供了理论基础。
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