Dantas Luíza L B, Calixto Cristiane P G, Dourado Maira M, Carneiro Monalisa S, Brown John W S, Hotta Carlos T
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
Division of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee, United Kingdom.
Front Plant Sci. 2019 Dec 23;10:1614. doi: 10.3389/fpls.2019.01614. eCollection 2019.
Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of intron-containing genes in , 46% in rice, and 38% in maize and it is widespread among the circadian clock genes. Little is known about how AS influences the circadian clock of C4 plants, like commercial sugarcane, a C4 crop with a complex hybrid genome. This work aims to test if the daily dynamics of AS forms of circadian clock genes are regulated by environmental factors, such as temperature, in the field. A systematic search for AS in five sugarcane clock genes, , , and using different organs of sugarcane sampled during winter, with 4 months old plants, and during summer, with 9 months old plants, revealed temperature- and organ-dependent expression of at least one alternatively spliced isoform in all genes. Expression of AS isoforms varied according to the season. Our results suggest that AS events in circadian clock genes are correlated with temperature.
可变剪接（Alternative Splicing，AS）是一种从同一基因的前体mRNA（pre-mRNA）产生不同成熟转录本的机制。在植物中，多种生理和代谢事件都与可变剪接有关，同时它也参与植物对温度变化的快速响应。可变剪接存在于约60%的含内含子基因中，在拟南芥中为46%，在水稻中为38%，在玉米中为38%，并且在生物钟基因中广泛存在。对于可变剪接如何影响C4植物的生物钟，人们了解甚少，例如商业甘蔗这种具有复杂杂交基因组的C4作物。这项研究旨在验证在田间条件下，生物钟基因可变剪接形式的日动态是否受温度等环境因素的调控。通过对甘蔗五个生物钟基因（[此处缺失具体基因名称]）进行系统搜索，利用冬季采样的4月龄甘蔗不同器官以及夏季采样的9月龄甘蔗不同器官，发现所有基因中至少有一种可变剪接异构体存在温度和器官依赖性表达。可变剪接异构体的表达随季节变化。我们的结果表明，生物钟基因中的可变剪接事件与温度相关。
