Center for Ecological Research, Kyoto University, Otsu, Shiga 520-2113, Japan.
Data Science and AI Innovation Research Promotion Center, Shiga University, Hikone, Shiga 522-8522, Japan.
Proc Natl Acad Sci U S A. 2024 Aug 27;121(35):e2402697121. doi: 10.1073/pnas.2402697121. Epub 2024 Aug 22.
Plants sense and respond to environmental cues during 24 h fluctuations in their environment. This requires the integration of internal cues such as circadian timing with environmental cues such as light and temperature to elicit cellular responses through signal transduction. However, the integration and transduction of circadian and environmental signals by plants growing in natural environments remains poorly understood. To gain insights into 24 h dynamics of environmental signaling in nature, we performed a field study of signal transduction from the nucleus to chloroplasts in a natural population of Using several modeling approaches to interpret the data, we identified that the circadian clock and temperature are key regulators of this pathway under natural conditions. We identified potential time-delay steps between pathway components, and diel fluctuations in the response of the pathway to temperature cues that are reminiscent of the process of circadian gating. We found that our modeling framework can be extended to other signaling pathways that undergo diel oscillations and respond to environmental cues. This approach of combining studies of gene expression in the field with modeling allowed us to identify the dynamic integration and transduction of environmental cues, in plant cells, under naturally fluctuating diel cycles.
植物在其环境中 24 小时波动时感知并响应环境线索。这需要将内部线索（如昼夜节律计时）与外部线索（如光和温度）整合在一起，通过信号转导引发细胞反应。然而，在自然环境中生长的植物对昼夜节律和环境信号的整合和转导仍然知之甚少。为了深入了解自然环境中环境信号的 24 小时动态，我们在一个自然种群中进行了从细胞核到叶绿体的信号转导的野外研究。利用几种建模方法来解释数据，我们发现昼夜节律钟和温度是自然条件下该途径的关键调节因子。我们确定了途径成分之间潜在的时间延迟步骤，以及途径对温度线索的反应的昼夜波动，这让人联想到昼夜节律门控的过程。我们发现，我们的建模框架可以扩展到其他经历昼夜振荡并响应环境线索的信号通路。这种将野外基因表达研究与建模相结合的方法使我们能够识别植物细胞在自然波动的昼夜周期下对环境线索的动态整合和转导。
