Kurisaki E, Kuroda Y, Sato M
Department of Legal Medicine, Central Research Laboratory, Fukushima Medical College, Japan.
Forensic Sci Int. 1988 Jul-Aug;38(1-2):3-11. doi: 10.1016/0379-0738(88)90003-5.
This paper presents a suicide case of copper sulfate ingestion. Post-mortem autopsy revealed mucous membrane necrosis of the esophagus and the stomach. Histological examination revealed centrilobular necrosis in the liver and renal insufficiency. The quantitative determination of copper, zinc and cadmium in various tissues showed that the copper concentrations in blood, liver, kidney and lung were 3.5-24-fold higher than those of the normal level, whereas zinc and cadmium concentrations were within normal range. Chromatographical patterns on Sephadex G-75 showed that most of the accumulated copper in the liver and kidney was bound to metallothionein (MT), a low molecular weight protein with high metal binding capacity which plays a role in the detoxification of heavy metals, while no copper bound to MT was found in the lung. These results suggest that the formation of Cu-induced MT in the liver and kidney occurred at the early stage in fatal acute copper poisoning.
本文介绍了一例硫酸铜摄入导致的自杀案例。尸检显示食管和胃黏膜坏死。组织学检查显示肝脏小叶中心坏死和肾功能不全。对各种组织中铜、锌和镉的定量测定表明，血液、肝脏、肾脏和肺中的铜浓度比正常水平高3.5至24倍，而锌和镉浓度在正常范围内。在Sephadex G - 75上的色谱图谱显示，肝脏和肾脏中积累的大部分铜与金属硫蛋白（MT）结合，金属硫蛋白是一种具有高金属结合能力的低分子量蛋白质，在重金属解毒中起作用，而在肺中未发现与MT结合的铜。这些结果表明，在致命性急性铜中毒的早期，肝脏和肾脏中Cu诱导的MT形成。
