Yahagi T, Nagao M, Seino Y, Matsushima T, Sugimura T
Mutat Res. 1977 Apr;48(2):121-9. doi: 10.1016/0027-5107(77)90151-8.
The mutagenic activities of 11 N-nitrosamines were tested using Salmonella typhimurium TA100 and TA98. All the carcinogenic N-nitrosamines were mutagenic on TA100 with a drug-activating system from the rat liver, whereas N,N-diphenylnitrosamine, a non-carcinogen, was not mutagenic. None of the N-nitrosamines was mutagenic on TA98, except N,N-diethylnitrosamine which was weakly mutagenic. To detect the mutagenicity of N,N-dimethylnitrosamine, the pre-incubation of bacteria and N,N-dimethylnitrosamine with S-9 Mix before if was poured onto plates was obligatorily required. Dimethyl sulfoxide inhibited the mutagenic effect of N,N-dimethylnitrosamine.
使用鼠伤寒沙门氏菌TA100和TA98测试了11种N-亚硝胺的致突变活性。所有致癌性N-亚硝胺在添加大鼠肝脏药物激活系统的情况下对TA100具有致突变性，而作为非致癌物的N,N-二苯基亚硝胺则无致突变性。除了具有弱致突变性的N,N-二乙基亚硝胺外，其他N-亚硝胺在TA98上均无致突变性。为检测N,N-二甲基亚硝胺的致突变性，必须在将细菌和N,N-二甲基亚硝胺与S-9混合物预孵育后再倾注到平板上。二甲基亚砜可抑制N,N-二甲基亚硝胺的致突变作用。
