Department of Pharmacology and Toxicology, Faculty of Pharmacy, Madonna University, Port Harcourt, Elele Rivers State, Nigeria.
Toxicology Unit, Faculty of Pharmacy, University of Port Harcourt, Port Harcourt Rivers State, Nigeria.
J Basic Clin Physiol Pharmacol. 2020 Jan 11;31(5):/j/jbcpp.2020.31.issue-5/jbcpp-2019-0188/jbcpp-2019-0188.xml. doi: 10.1515/jbcpp-2019-0188.
Pleurotus tuber regium, a wild edible mushroom can reduce free radical-mediated injury and oxidative stress induced by carbon tetrachloride (CCl4) via improvement of antioxidant capacity. This work evaluates the protective effects of this mushroom against the metabolic and hematological toxicity induced by CCl4. Sixty male Sprague Dawley rats were divided into six groups (n = 10). Group I received olive oil (3 mL/kg) i.p. twice weekly for 13 weeks, while maintaining free access to food and water ad libitum (negative control). Group II received 3 mL/kg (30% CCl4 in olive oil) injected i.p. twice weekly, while Groups III, IV, and V received 100, 200, and 500 mg wild edible P. tuber regium (33.3% in feed) daily in addition to 3 mL/kg CCl4 in olive oil injected twice weekly i.p. Group VI received olive oil (3 mL/kg) i.p. twice weekly for 13 weeks in addition to 500 mg P. tuber regium (33.3% in feed) daily. The body weight (b.w.), feed intake (FI), and water intake (WI) were obtained weekly, while the hematological indices and oxidative stress parameters were carried out shortly after necropsy on days 30, 60, and 90. Treatment with CCl4 significantly (p < 0.05) decreased the b.w., FI and WI, feed efficiency, ascorbic acid, α-tocopherol, and antioxidant enzymes, superoxide dismutase, catalase, total glutathione, and peroxidase, while increasing the oxidative stress as measured by malondialdehyde in CCl4 only group when compared with control. Supplementation of feed with P. tuber regium reversed the effects of CCl4. Pleurotus tuber regium ameliorated the CCl4-induced metabolic and hematotoxicity by improving the antioxidant capacity.
美味牛肝菌，一种野生食用蘑菇，可以通过提高抗氧化能力来减少四氯化碳（CCl4）引起的自由基介导的损伤和氧化应激。本研究评估了该蘑菇对 CCl4 引起的代谢和血液毒性的保护作用。60 只雄性 Sprague Dawley 大鼠被分为 6 组（n = 10）。第 I 组每周两次腹腔注射橄榄油（3 mL/kg），共 13 周，同时自由摄取食物和水（阴性对照）。第 II 组每周两次腹腔注射 3 mL/kg（30% CCl4 在橄榄油中），而第 III、IV 和 V 组分别每周两次腹腔注射 3 mL/kg CCl4 ，同时每天还接受 100、200 和 500 mg 野生食用美味牛肝菌（饲料中 33.3%）。第 VI 组每周两次腹腔注射橄榄油（3 mL/kg），共 13 周，同时每天还接受 500 mg 美味牛肝菌（饲料中 33.3%）。每周测量体重（b.w.）、采食量（FI）和饮水量（WI），而在第 30、60 和 90 天解剖后立即进行血液学指标和氧化应激参数的检测。与对照组相比，CCl4 处理显著（p < 0.05）降低了 b.w.、FI 和 WI、饲料效率、抗坏血酸、α-生育酚和抗氧化酶、超氧化物歧化酶、过氧化氢酶、总谷胱甘肽和过氧化物酶，而丙二醛等氧化应激标志物在 CCl4 组中增加。用美味牛肝菌补充饲料逆转了 CCl4 的作用。美味牛肝菌通过提高抗氧化能力改善了 CCl4 引起的代谢和血液毒性。
