Department of Biology, The Faculty of Science and Literature, Inonu University, Malatya, Turkey.
Mol Biol Rep. 2020 Mar;47(3):1667-1680. doi: 10.1007/s11033-020-05256-2. Epub 2020 Jan 13.
Obesity is a complex, chronic disease that arises according to the interaction between genetic and environmental factors. The expansion and growth of white adipose tissue (WAT) could be related to angiogenesis. Resveratrol and adrenomedullin (AdM) were used for the inhibition of angiogenesis in metabolically passive WAT for inhibiting the expansion of this tissue, and the activation of angiogenesis in metabolically active brown adipose tissue (BAT) for increasing daily energy consumption as a way of reducing obesity. Rats were divided into eight groups. Four obese groups were fed with a high-fat diet containing 60% fat as energy for three months. After obtaining obesity, 2.5 nmol/kg AdM and 10 mg/kg resveratrol were treated to experiment groups intraperitoneally (i.p.) every other day for four weeks. AdM and vascular endothelial growth factor A (VEGF-A) mRNA levels were detected with semi-quantitative PCR; protein levels were detected with Western Blotting. AdM and resveratrol are multifactorial molecules, thus, this study has revealed a few novel evidence. The results were distinct in the group and treatment levels. The results showed that resveratrol has a role in angiogenesis in obesity and contributed to AdM production. It is observed that AdM has regulated its expression and increased the effect of resveratrol in WAT. AdM and VEGF-A gene expressions could not be detected in BAT; however, it is suggested that resveratrol may have a pro-angiogenic effect in BAT of obese rats according to the protein levels. AdM also has regulated VEGF-A level according to the metabolic situation of the organism.
肥胖是一种复杂的慢性疾病，是由遗传和环境因素相互作用引起的。白色脂肪组织（WAT）的扩张和生长可能与血管生成有关。白藜芦醇和肾上腺髓质素（AdM）被用于抑制代谢不活跃的 WAT 中的血管生成，以抑制该组织的扩张，并激活代谢活跃的棕色脂肪组织（BAT）中的血管生成，以增加每日能量消耗，从而减少肥胖。大鼠被分为 8 组。四组肥胖大鼠喂食含 60%脂肪作为能量的高脂肪饮食三个月。获得肥胖后，每天腹腔内（i.p.）注射 2.5 nmol/kg AdM 和 10 mg/kg 白藜芦醇，共 4 周。用半定量 PCR 检测 AdM 和血管内皮生长因子 A（VEGF-A）mRNA 水平；用 Western Blotting 检测蛋白水平。AdM 和白藜芦醇是多因素分子，因此，本研究揭示了一些新的证据。结果在组和处理水平上是不同的。结果表明，白藜芦醇在肥胖中的血管生成中起作用，并有助于 AdM 的产生。观察到 AdM 调节了其表达，并增强了白藜芦醇在 WAT 中的作用。BAT 中未检测到 AdM 和 VEGF-A 基因表达；然而，根据蛋白水平，提示白藜芦醇可能对肥胖大鼠的 BAT 具有促血管生成作用。AdM 根据机体的代谢情况调节 VEGF-A 水平。
