食管鳞状细胞癌患者中CK1α与β-连环蛋白Ser45磷酸化之间的相关性

Correlation between CK1α and β-catenin Ser45-phosphorylation in patients with esophageal squamous cell carcinoma.

作者信息

Pan Xinning, Ma Lili, Wang Jinsheng

机构信息

Department of Pathology, Changzhi Medical College Changzhi, Shanxi, China.

Department of Pathology, Heping Hospital Affiliated to Changzhi Medical College Changzhi, Shanxi, China.

出版信息

Int J Clin Exp Pathol. 2019 Oct 1;12(10):3928-3933. eCollection 2019.

PMID:31933784

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6949772/

Abstract

UNLABELLED

Wnt/β-catenin signaling plays important role in development and tumorigenesis. The accumulation of β-catenin in cytoplasm/nucleus leads to many diseases including cancer. In the normal cells, β-catenin is phosphorylated by CKIα and GSK-3, then phosphorylated β-catenin is sequentially degraded. However, the exact role and correlation of CK1α and β-catenin Ser45-phosphorylation in esophageal squamous cell carcinoma (ESCC) remains little known.

PURPOSE

The present study was aimed at exploring the role and the correlation between the expression of CK1α and β-catenin Ser45-phosphorylation in ESCC.

METHODS

The expression of CK1α and β-catenin Ser45-phosphorylation were detected by immunohistochemical technique in 90 cases of ESCC and their corresponding adjacent nonneoplastic esophageal tissues (n=90), and evaluated the correlation between the expression of CK1α, phosphorylation at Ser45 of β-catenin and clinicopathological parameters of ESCC patients.

RESULTS

The lever of the expression of CK1α in ESCC was 63.3% (57/90), significantly lower than that in nonneoplastic esophageal tissues was 84.4% (76/90), (X=16.567, P=0.000). The lever of the expression of phosphorylation at Ser45 of β-catenin in ESCC was 65.6% (59/90), significantly lower than that in nonneoplastic esophageal tissues was 88.9% (80/90), (X=10.340, P=0.003). The expression of CK1α and β-catenin Ser45-phosphorylation was significantly related to the degree of tumor cell differentiation, but not with age, gender, tumor size, AJCC clinical stage and lymphatic metastasis. And CK1α and β-catenin Ser45-phosphorylation expression were also positively correlated (0.356, P=0.001).

CONCLUSION

CK1α and β-catenin Ser45-phosphorylation may play an important role in the pathogenesis and development of ESCC, and provide clinically useful information.

摘要

未标记

Wnt/β-连环蛋白信号通路在发育和肿瘤发生中起重要作用。β-连环蛋白在细胞质/细胞核中的积累会导致包括癌症在内的多种疾病。在正常细胞中，β-连环蛋白被CKIα和GSK-3磷酸化，然后磷酸化的β-连环蛋白被依次降解。然而，CK1α和β-连环蛋白Ser45磷酸化在食管鳞状细胞癌（ESCC）中的确切作用及相关性仍鲜为人知。

目的

本研究旨在探讨CK1α表达与β-连环蛋白Ser45磷酸化在ESCC中的作用及相关性。

方法

采用免疫组织化学技术检测90例ESCC及其相应的癌旁非肿瘤食管组织（n = 90）中CK1α和β-连环蛋白Ser45磷酸化的表达，并评估CK1α表达、β-连环蛋白Ser45磷酸化与ESCC患者临床病理参数之间的相关性。

结果

ESCC中CK1α的表达水平为63.3%（57/90），显著低于非肿瘤食管组织中的84.4%（76/90），（X = 16.567，P = 0.000）。ESCC中β-连环蛋白Ser45磷酸化的表达水平为65.6%（59/90），显著低于非肿瘤食管组织中的88.9%（80/90），（X = 10.340，P = 0.003）。CK1α和β-连环蛋白Ser45磷酸化的表达与肿瘤细胞分化程度显著相关，但与年龄、性别、肿瘤大小、AJCC临床分期及淋巴转移无关。且CK1α与β-连环蛋白Ser45磷酸化表达也呈正相关（0.356，P = 0.001）。