Mengfan Qiao, Xifeng Wang, Guowu Zhang, Qingling Meng, Jun Qiao, Lixia Wang, Kuojun Cai, Jinsheng Zhang, Zaichao Zhang, Weiwei Yu, Yelong Peng, Xuepeng Cai
College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, China.
School of Biotechnology, Central South University, Changsha, Hunan, 410012, China.
J Vet Res. 2019 Dec 19;63(4):481-488. doi: 10.2478/jvetres-2019-0071. eCollection 2019 Dec.
type 3 (PCV3) is a newly discovered . The molecular characteristics and genetic evolution of PCV3 in Xinjiang province, China still being unclear, the aim of the study was their elucidation.
A total of 393 clinical samples were collected from pigs on commercial farms in nine different regions of Xinjiang and phylogenetic analysis based on full-length genes was performed.
The prevalence at farm level was 100%, while in all the tested samples it was 22.39%. Nine PCV3 strains were detected in Xinjiang province and they shared 98.9-99.3% nucleotide and 97.5-100.0% gene amino acid sequence identities with other epidemic strains from China and abroad. Compared with other epidemic strains of PCV3, there were 26 base mutation sites in the gene in the nine Xinjiang strains, resulting in the mutation of amino acids at positions 20, 24, 75, 77, 108, 111 and 206. Phylogenetic analysis showed that these strains can be divided into two different genetic groups, to the first of which five strains affiliated and divided between subgroups 1.1 and 1.2, and to the second of which the other four strains affiliated and similarly divided between subgroups 2.1 and 2.2.
PCV3 circulates widely among commercial pig farms in Xinjiang province, China, and displays obvious genetic diversity. The results provide epidemiological information useful for the prevention and control of PCV3 infection in the pig industry.
3型猪圆环病毒（PCV3）是一种新发现的病毒。中国新疆地区PCV3的分子特征和基因进化仍不清楚，本研究旨在阐明这些问题。
从新疆9个不同地区的商业猪场采集了393份猪临床样本，并基于全长基因进行了系统发育分析。
猪场水平的流行率为100%，而在所有检测样本中为22.39%。在新疆地区检测到9株PCV3毒株，它们与来自中国和国外的其他流行毒株的核苷酸序列同一性为98.9-99.3%，基因氨基酸序列同一性为97.5-100.0%。与其他PCV3流行毒株相比，9株新疆毒株的基因中有26个碱基突变位点，导致第20、24、75、77、108、111和206位氨基酸发生突变。系统发育分析表明，这些毒株可分为两个不同的遗传组，其中5株属于第一组，在1.1和1.2亚组之间划分，另外4株属于第二组，在2.1和2.2亚组之间类似地划分。
PCV3在中国新疆地区的商业猪场中广泛传播，并表现出明显的遗传多样性。这些结果为猪业中PCV3感染的预防和控制提供了有用的流行病学信息。
