Zhengzhou Key Laboratory for pig disease prevention and control, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Zhengzhou Major Pig Disease Prevention and Control Laboratory, Zhengzhou, China.
Transbound Emerg Dis. 2018 Oct;65(5):1163-1169. doi: 10.1111/tbed.12920. Epub 2018 Jun 7.
Porcine circovirus type 3 (PCV3) is the pathogen responsible for a new infectious disease that was first reported in 2016 in the United States. To further investigate the epidemic profile and genetic diversity of the virus, one hundred and seventy clinical samples (110 tissue samples and 60 serum samples) were collected from 41 different pig farms in 14 cities in central China, and a SYBR Green I-based quantitative real-time PCR method was developed to detect PCV3. The partial cap genes of four field strains from four different farms were sequenced and analysed. The results showed the detection limit was 2.19 × 10 genome copies/μl. Fifty-three of 170 samples were detected as positive for PCV3, giving a PCV3-positive rate of 31.18%, with 48.78% (20/41) of pig farms harbouring PCV3, which varied from 20% to 42.86% between 2013 and 2017. PCV3 could be detected in samples from pigs with different clinical presentations, and the PCV3-positive rates varied for these different clinical presentations. The partial capsid genes of four PCV3 strains (designated YZ, LY-03, NY and SP) shared 96.3%-99.4% nucleotide identity with those available in GenBank. Phylogenetic analysis based on the capsid gene of 32 PCV3 strains showed that the four PCV3 strains in this study were clustered with the China/GD2016 and South Korea Ku-1606 strains. The results of this study will aid our understanding of the molecular epidemiology of PCV3.
猪圆环病毒 3 型（PCV3）是一种新传染病的病原体，该传染病于 2016 年首次在美国报道。为了进一步研究该病毒的流行情况和遗传多样性，从中国中部 14 个城市的 41 个不同猪场采集了 170 份临床样本（110 份组织样本和 60 份血清样本），并建立了基于 SYBR Green I 的实时定量 PCR 方法来检测 PCV3。对来自四个不同农场的四个田间毒株的部分 cap 基因进行了测序和分析。结果表明，检测限为 2.19×10 基因组拷贝/μl。170 个样本中有 53 个被检测为 PCV3 阳性，阳性率为 31.18%，48.78%（20/41）的猪场携带 PCV3，2013 年至 2017 年之间的阳性率在 20%至 42.86%之间变化。PCV3 可在具有不同临床表现的猪的样本中检测到，并且这些不同临床表现的 PCV3 阳性率不同。四个 PCV3 株（命名为 YZ、LY-03、NY 和 SP）的部分衣壳基因与 GenBank 中可用的基因核苷酸同一性为 96.3%-99.4%。基于 32 个 PCV3 株的衣壳基因的系统进化分析表明，本研究中的四个 PCV3 株与中国/GD2016 和韩国 Ku-1606 株聚类。本研究的结果将有助于我们了解 PCV3 的分子流行病学。
