Pau Salvatore, Falchi Laura, Ledda Mauro, Pivato Ivo, Valentino Melosu, Bogliolo Luisa, Ariu Federica, Zedda Maria Teresa
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna n.2, 07100 Sassari, Italy.
Centro di Competenza Biodiversità Animale, viale Adua 2/c, 07100 Sassari, Italy.
Animals (Basel). 2020 Jan 9;10(1):108. doi: 10.3390/ani10010108.
Transcervical artificial insemination (AI) after the surgical incision of cervical folds (SICF) could represent a valid alternative to laparoscopic AI when frozen thawed semen is used. The aim of this experiment was to compare pregnancy (PR) and lambing rates (LR) of ewes submitted either to transcervical AI after SICF or to laparoscopic AI using frozen thawed semen. Pregnant at term ewes (n = 80) were allocated in two experimental groups. After lambing, one group (n = 39) was submitted to SICF. The remaining ewes that were regularly lambed were allocated to the group of laparoscopic AI (n = 40). Six months later, oestrous cycle of both experimental groups was synchronised and all ewes were artificially inseminated with frozen thawed semen. Ewes submitted to SICF underwent transcervical insemination and intrauterine deposition of semen was recorded. The remaining animals were submitted to laparoscopic AI. Pregnancy and LR were recorded. Intrauterine deposition of semen was possible in 89.7% pf ewes submitted to SICF. This group showed similar PR and LR compared to the laparoscopic group (respectively: PR, 71.8% vs. 70% and LR, 64.1% vs. 65%; > 0.05). Transcervical AI after SICF may represent a valid alternative to laparoscopy in AI protocols requiring the use of frozen thawed semen.
当使用冻融精液时,在宫颈皱襞手术切开(SICF)后进行经宫颈人工授精(AI)可能是腹腔镜人工授精的有效替代方法。本实验的目的是比较接受SICF后经宫颈人工授精或使用冻融精液进行腹腔镜人工授精的母羊的妊娠率(PR)和产羔率(LR)。足月妊娠母羊(n = 80)被分配到两个实验组。产羔后,一组(n = 39)接受SICF。其余正常产羔的母羊被分配到腹腔镜人工授精组(n = 40)。六个月后,同步两个实验组的发情周期,所有母羊均用冻融精液进行人工授精。接受SICF的母羊进行经宫颈授精,并记录精液的子宫内沉积情况。其余动物接受腹腔镜人工授精。记录妊娠情况和产羔率。在接受SICF的母羊中,89.7%的母羊实现了精液的子宫内沉积。与腹腔镜组相比,该组的妊娠率和产羔率相似(分别为:妊娠率,71.8%对70%;产羔率,64.1%对65%;P>0.05)。在需要使用冻融精液的人工授精方案中,SICF后的经宫颈人工授精可能是腹腔镜检查的有效替代方法。