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本文引用的文献

1
Surgery on cervical folds for transcervical intrauterine artificial insemination with frozen-thawed semen enhances pregnancy rates in the sheep.经宫颈子宫内冻融精液人工授精时对子宫颈皱襞进行手术可提高绵羊的妊娠率。
Theriogenology. 2019 Mar 1;126:28-35. doi: 10.1016/j.theriogenology.2018.11.019. Epub 2018 Nov 22.
2
Laparoscopic Artificial Insemination Technique in Small Ruminants-A Procedure Review.小型反刍动物的腹腔镜人工授精技术——手术综述
Front Vet Sci. 2018 Oct 23;5:266. doi: 10.3389/fvets.2018.00266. eCollection 2018.
3
Technical Note: A new device for cervical insemination of sheep - design and field test.技术说明:一种绵羊宫颈内人工授精的新装置——设计与田间试验。
J Anim Sci. 2017 Dec;95(12):5263-5269. doi: 10.2527/jas2017.1951.
4
Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen.绵羊采用新鲜精液和冻融精液人工授精方法的繁殖反应。
Cryobiology. 2017 Feb;74:77-80. doi: 10.1016/j.cryobiol.2016.11.012. Epub 2016 Nov 29.
5
Assessing the usefulness of prostaglandin E2 (Cervidil) for transcervical artificial insemination in ewes.评估前列腺素E2(地诺前列酮栓)在母羊经宫颈人工授精中的效用。
Theriogenology. 2015 Dec;84(9):1594-602. doi: 10.1016/j.theriogenology.2015.08.007. Epub 2015 Aug 24.
6
The pattern of cervical penetration and the effect of topical treatment with prostaglandin and/or FSH and oxytocin on the depth of cervical penetration in the ewe during the peri-ovulatory period.在绵羊的排卵周期中,宫颈穿透的模式以及前列腺素和/或 FSH 和催产素的局部处理对宫颈穿透深度的影响。
Theriogenology. 2012 Jul 15;78(2):376-84. doi: 10.1016/j.theriogenology.2012.02.017. Epub 2012 Apr 24.
7
Design and "in vivo" evaluation of two adapted catheters for intrauterine transcervical insemination in sheep.设计并“体内”评估两种改良的羊子宫颈内授精用导管。
Anim Reprod Sci. 2012 Apr;131(3-4):153-9. doi: 10.1016/j.anireprosci.2012.03.001. Epub 2012 Mar 10.
8
Fertility after different artificial insemination methods using a synthetic semen extender in sheep.在绵羊中使用合成精液稀释剂的不同人工授精方法后的繁殖力。
J Reprod Dev. 2009 Feb;55(1):50-4. doi: 10.1262/jrd.20062. Epub 2008 Nov 10.
9
The effects of the prostaglandin E analogue Misoprostol and follicle-stimulating hormone on cervical penetrability in ewes during the peri-ovulatory period.前列腺素E类似物米索前列醇和促卵泡激素对排卵期前后母羊子宫颈通透性的影响。
Theriogenology. 2007 Mar 1;67(4):767-77. doi: 10.1016/j.theriogenology.2006.10.012. Epub 2006 Nov 28.
10
Influence of breed and age on morphometry and depth of inseminating catheter penetration in the ewe cervix: a postmortem study.品种和年龄对母羊子宫颈形态测量及授精导管插入深度的影响:一项死后研究
Theriogenology. 2006 Nov;66(8):1876-83. doi: 10.1016/j.theriogenology.2006.04.039. Epub 2006 Jun 21.

对接受子宫颈皱襞手术切口(SICF)的母羊进行经宫颈冷冻解冻精液授精后的繁殖性能:与腹腔镜人工授精的比较。

Reproductive Performance Following Transcervical Insemination with Frozen Thawed Semen in Ewes Submitted to Surgical Incision of Cervical Folds (SICF): Comparison with Laparoscopic Artificial Insemination.

作者信息

Pau Salvatore, Falchi Laura, Ledda Mauro, Pivato Ivo, Valentino Melosu, Bogliolo Luisa, Ariu Federica, Zedda Maria Teresa

机构信息

Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna n.2, 07100 Sassari, Italy.

Centro di Competenza Biodiversità Animale, viale Adua 2/c, 07100 Sassari, Italy.

出版信息

Animals (Basel). 2020 Jan 9;10(1):108. doi: 10.3390/ani10010108.

DOI:10.3390/ani10010108
PMID:31936444
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7022337/
Abstract

Transcervical artificial insemination (AI) after the surgical incision of cervical folds (SICF) could represent a valid alternative to laparoscopic AI when frozen thawed semen is used. The aim of this experiment was to compare pregnancy (PR) and lambing rates (LR) of ewes submitted either to transcervical AI after SICF or to laparoscopic AI using frozen thawed semen. Pregnant at term ewes (n = 80) were allocated in two experimental groups. After lambing, one group (n = 39) was submitted to SICF. The remaining ewes that were regularly lambed were allocated to the group of laparoscopic AI (n = 40). Six months later, oestrous cycle of both experimental groups was synchronised and all ewes were artificially inseminated with frozen thawed semen. Ewes submitted to SICF underwent transcervical insemination and intrauterine deposition of semen was recorded. The remaining animals were submitted to laparoscopic AI. Pregnancy and LR were recorded. Intrauterine deposition of semen was possible in 89.7% pf ewes submitted to SICF. This group showed similar PR and LR compared to the laparoscopic group (respectively: PR, 71.8% vs. 70% and LR, 64.1% vs. 65%; > 0.05). Transcervical AI after SICF may represent a valid alternative to laparoscopy in AI protocols requiring the use of frozen thawed semen.

摘要

当使用冻融精液时,在宫颈皱襞手术切开(SICF)后进行经宫颈人工授精(AI)可能是腹腔镜人工授精的有效替代方法。本实验的目的是比较接受SICF后经宫颈人工授精或使用冻融精液进行腹腔镜人工授精的母羊的妊娠率(PR)和产羔率(LR)。足月妊娠母羊(n = 80)被分配到两个实验组。产羔后,一组(n = 39)接受SICF。其余正常产羔的母羊被分配到腹腔镜人工授精组(n = 40)。六个月后,同步两个实验组的发情周期,所有母羊均用冻融精液进行人工授精。接受SICF的母羊进行经宫颈授精,并记录精液的子宫内沉积情况。其余动物接受腹腔镜人工授精。记录妊娠情况和产羔率。在接受SICF的母羊中,89.7%的母羊实现了精液的子宫内沉积。与腹腔镜组相比,该组的妊娠率和产羔率相似(分别为:妊娠率,71.8%对70%;产羔率,64.1%对65%;P>0.05)。在需要使用冻融精液的人工授精方案中,SICF后的经宫颈人工授精可能是腹腔镜检查的有效替代方法。