Falchi Laura, Zedda Maria Teresa, Pau Salvatore, Ledda Mauro, Melosu Valentino, Rassu Salvatore Pier Giacomo
Sezione di Clinica Ostetrica e Ginecologia, Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Via Vienna n. 2, 07100 Sassari, Italy.
Sezione di Scienze Zootecniche, Dipartimento di Agraria, Università degli Studi di Sassari, Viale Italia n. 39, 07100 Sassari, Italy.
Animals (Basel). 2021 Nov 23;11(12):3348. doi: 10.3390/ani11123348.
In ovine species, transcervical artificial insemination (TCAI) is limited by the poor quality of frozen-thawed semen and by the convoluted cervical lumen hampering the passage of inseminating devices. The aim of the study was to test the efficiency of three newly designed catheters with bent tips of 3.5 mm, 5.0 mm or 8.0 mm in terms of reproductive performances (experiment 1) and to compare the results of TCAI with the best performing catheter of experiment 1 to those obtained in ewes submitted to surgical incision of cervical folds (SICF) prior to insemination (experiment 2). The following parameters were assessed: time to pass the cervix; depth of cervical penetration; site of deposition of semen; pregnancy (PR); and lambing rates (LR). The results of experiment 1 indicated that the 5.0 mm tip catheter resulted in deeper and faster TCAI and higher PR and LR compared to 3.5 mm and 8.0 mm tip catheters ( < 0.05). In experiment 2, TCAI with the 5.0 mm catheter did not differ from TCAI after SICF in terms of depth of semen deposition, time to pass the cervix, PR and LR ( < 0.05). In conclusion, the use of a catheter that allowed transcervical uterine deposition of semen without excessive manipulation led to satisfactory pregnancy rates.
在绵羊物种中，经颈人工授精（TCAI）受到冻融精液质量差以及宫颈管腔曲折妨碍授精器械通过的限制。本研究的目的是测试三种新设计的尖端弯曲分别为3.5毫米、5.0毫米或8.0毫米的导管在生殖性能方面的效率（实验1），并将实验1中性能最佳的导管进行TCAI的结果与在授精前对母羊进行宫颈皱襞手术切口（SICF）所获得的结果进行比较（实验2）。评估了以下参数：通过宫颈的时间；宫颈穿透深度；精液沉积部位；妊娠率（PR）；以及产羔率（LR）。实验1的结果表明，与尖端为3.5毫米和8.0毫米的导管相比，尖端为5.0毫米的导管导致更深更快的TCAI以及更高的PR和LR（<0.05）。在实验2中，使用5.0毫米导管进行TCAI在精液沉积深度、通过宫颈的时间、PR和LR方面与SICF后的TCAI没有差异（<0.05）。总之，使用一种无需过多操作就能经宫颈将精液沉积到子宫的导管可获得令人满意的妊娠率。
