College of Horticulture, Northwest A&F University, Yangling 712100, China.
Guizhou Institute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China.
Int J Mol Sci. 2020 Jan 10;21(2):444. doi: 10.3390/ijms21020444.
SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction, and stress response. However, little is known about the role of pepper SBP-box transcription factor genes in response to abiotic stress. Here, one of the pepper SBP-box gene, , was selected and isolated from pepper genome database in our previous study. The gene was induced under salt stress. Silencing the gene enhanced pepper plant tolerance to salt stress. The accumulation of reactive oxygen species (ROS) of the detached leaves of -silenced plants was significantly lower than that of control plants. Besides, the Na, malondialdehyde content, and conductivity were significantly increased in control plants than that in the -silenced plants. In addition, the over-expressed plants were more susceptible to salt stress with higher damage severity index percentage and accumulation of ROS as compared to the wild-type. These results indicated that negatively regulates salt stress tolerance in pepper may relate to ROS signaling cascades.
SBP-box（Squamosa-promoter binding protein）基因是一类植物特异性转录因子，在植物生长、信号转导和应激反应中发挥重要作用。然而，关于辣椒 SBP-box 转录因子基因在应对非生物胁迫中的作用知之甚少。在本研究中，我们从辣椒基因组数据库中选择并分离了一个辣椒 SBP-box 基因，命名为 。该基因受盐胁迫诱导。沉默 基因增强了辣椒植株对盐胁迫的耐受性。-沉默植株的离体叶片中活性氧（ROS）的积累明显低于对照植株。此外，对照植株中的 Na+、丙二醛含量和电导率均明显高于 -沉默植株。此外，与野生型相比，过表达 植株对盐胁迫更敏感，ROS 积累和损伤严重指数百分比更高。这些结果表明， 可能通过 ROS 信号级联负调控辣椒的耐盐性。
