DKK3启动子的甲基化与宫颈腺癌患者的不良预后相关。
Methylation of the DKK3 promoter is associated with poor prognosis in patients with cervical adenocarcinoma.
作者信息
Zhang Min, Huang Minna, Cao Baoli, Sheng Xiaoying, Li Ping
机构信息
Nankai Hospital Tianjin, China.
Department of Oncology, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin, China.
出版信息
Int J Clin Exp Pathol. 2018 Feb 1;11(2):788-794. eCollection 2018.
OBJECTIVE
The aim of this study was to investigate the mRNA of DKK3 (Dickkopf-3) in cervical adenocarcinoma, and to explore correlations between methylation status of the DKK3 promoter and biological behaviors of cervical adenocarcinoma.
METHODS
The mRNA expression level of DKK3 was detected by real-time quantitative reverse transcription PCR. Methylation-specific PCR (MSP) analysis was performed to detect the methylated degrees of the DNA of the DKK3 promoter.
RESULTS
The mRNA expression levels of DKK3 in cervical adenocarcinoma tissues were lower than those in adjacent normal cervical tissues. MSP detection found DKK3 promoter methylation was 38% in cervical adenocarcinoma tissues, while no normal cervical tissues were found to be methylated.FIGO staging and pelvic lymph node metastasis were identified as relative factors of methylation status of the DKK3 promoter. Multivariate analysis demonstrated methylation status of the DKK3 promoter was an independent prognostic indicator of cervical adenocarcinoma. Patients with methylated DKK3 promoter exhibited significantly shorter OS than those with an unmethylated DKK3 promoter.
CONCLUSIONS
The methylation status of the DKK3 promoter may indicate poor prognosis of patients with cervical adenocarcinoma.
目的
本研究旨在检测DKK3（Dickkopf-3）在宫颈腺癌中的信使核糖核酸（mRNA），并探讨DKK3启动子甲基化状态与宫颈腺癌生物学行为之间的相关性。
方法
采用实时定量逆转录聚合酶链反应检测DKK3的mRNA表达水平。进行甲基化特异性聚合酶链反应（MSP）分析以检测DKK3启动子DNA的甲基化程度。
结果
宫颈腺癌组织中DKK3的mRNA表达水平低于相邻正常宫颈组织。MSP检测发现宫颈腺癌组织中DKK3启动子甲基化率为38%，而未发现正常宫颈组织存在甲基化。国际妇产科联盟（FIGO）分期和盆腔淋巴结转移被确定为DKK3启动子甲基化状态的相关因素。多因素分析表明，DKK3启动子甲基化状态是宫颈腺癌的独立预后指标。DKK3启动子甲基化的患者总生存期明显短于DKK3启动子未甲基化的患者。
结论
DKK3启动子甲基化状态可能提示宫颈腺癌患者预后不良。
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