Spivak V A, Lutsenko I N, Aseeva E A
Mol Gen Mikrobiol Virusol. 1988 Aug(8):46-8.
A new strategy for structural identification of abnormal human hemoglobins is proposed. It is based on micropreparative modification of electrophoretic separation of globins on Cellogel strips with subsequent quantitative isolation of a pure, desalted globin chain, in a form suitable for its subsequent structural investigation. Among the major advantages of the new strategy age possibility to use small blood samples (0.1-0.2 ml), short analysis time, relative simplicity and low cost.
提出了一种鉴定异常人类血红蛋白结构的新策略。该策略基于对Cellogel条带上珠蛋白进行电泳分离的微量制备修饰,随后定量分离出适合后续结构研究的纯的、脱盐的珠蛋白链。新策略的主要优点包括能够使用少量血液样本(0.1 - 0.2毫升)、分析时间短、相对简单且成本低。