Steiman I, Norwood C, Charache S
J Lab Clin Med. 1985 Mar;105(3):328-30.
Measurement of the proportion of abnormal hemoglobin in a hemolysate is essential for differentiation of simple and compound heterozygotes (e.g., sickle cell trait vs. sickle thalassemia), for differential of alpha- and beta-thalassemia in compound heterozygotes, and for differentiation of various types of beta-thalassemia in such persons. Utilization of such measurements is hampered by the imprecision and inconvenience of current methods. We have adapted a readily available agar electrophoresis method for this purpose, scanning unstained gels at 420 nm. The new method is particularly valuable for rapid estimation of percent of HbS after partial exchange transfusion in patients with sickle cell anemia. It cannot be used for quantitation of HbF or for quantitation of hemoglobins that comigrate with HbA; contrariwise, it can be used for hemoglobins that only separate from HbA on agar (e.g., HbBethesda).
测定溶血产物中异常血红蛋白的比例对于区分单纯杂合子和复合杂合子（如镰状细胞性状与镰状细胞地中海贫血）、区分复合杂合子中的α地中海贫血和β地中海贫血以及区分此类患者中各种类型的β地中海贫血至关重要。目前方法的不精确性和不便性阻碍了此类测量的应用。我们为此采用了一种现成的琼脂电泳方法，在420nm波长下扫描未染色的凝胶。这种新方法对于快速估算镰状细胞贫血患者部分换血后HbS的百分比特别有价值。它不能用于定量HbF或与HbA共迁移的血红蛋白；相反，它可用于仅在琼脂上与HbA分离的血红蛋白（如Hb贝塞斯达）。
