在异常人类血红蛋白的结构鉴定中，利用醋酸纤维素薄膜对珠蛋白链进行微量制备电泳。
Micropreparative electrophoresis of globin chains on cellulose acetate film in structural identification of abnormal human hemoglobins.
作者信息
Spivak V A, Lutsenko I N
机构信息
Central Research Institute of Hematology and Blood Transfusion, Moscow, USSR.
出版信息
Anal Biochem. 1988 May 15;171(1):108-12. doi: 10.1016/0003-2697(88)90130-3.
A simple and rapid micropreparative method for isolating 5 to 30 nmol of globin chain, followed by structural identification of abnormal human hemoglobins, is described. The method is based on the electrophoretic separation of globins on ordinary Cellogel films under denaturating conditions with subsequent cutting out of the protein zones and solubilization of Cellogel and the electrophoretic buffer components in a specially selected solvent in which the globin chain undergoes quantitative precipitation. The method makes it possible to simplify and speed up the structural identification of commonly occurring abnormal hemoglobins. The advantages and limitations of the method are discussed along with its potential uses in structural protein chemistry.
本文描述了一种简单快速的微量制备方法，用于分离5至30纳摩尔的珠蛋白链，随后对异常人类血红蛋白进行结构鉴定。该方法基于在变性条件下，在普通Cellogel薄膜上对珠蛋白进行电泳分离，随后切下蛋白质区带，并将Cellogel和电泳缓冲液成分溶解在一种特别选择的溶剂中，珠蛋白链在该溶剂中会发生定量沉淀。该方法能够简化并加速常见异常血红蛋白的结构鉴定。文中讨论了该方法的优缺点及其在结构蛋白质化学中的潜在用途。
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