Laboratory for Proteomics and Metabolomics, Research Division, General Hospital of Mexico "Dr. Eduardo Liceaga," Mexico City, Mexico.
Immunogenomics and Metabolic Diseases Laboratory, Instituto Nacional de Medicina Genómica, SS, Mexico City, Mexico.
J Interferon Cytokine Res. 2020 Mar;40(3):131-138. doi: 10.1089/jir.2019.0149. Epub 2020 Jan 16.
There is scant information regarding the role of interleukin (IL)-6 in obesity-related metabolic dysfunction in humans. Thus, we studied the serum levels of IL-6 in normal weight, overweight, and obese subjects, and examined associations of IL-6 with hyperglycemia, insulin resistance, dyslipidemia, and systemic inflammation. One hundred three women and men were included in the study. Anthropometric parameters, blood glucose, insulin, total cholesterol, and triglycerides were measured. Serum levels of tumor necrosis factor-alpha (TNF-alpha), IL-10, and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance (ANOVA) showed a 2.5-fold significant decrease in serum IL-6 in overweight and obese individuals when compared with normal weight controls. Serum IL-6 exhibited significant inverse correlations with body mass index ( = -0.39/ < 0.0001), waist circumference ( = -0.42/ < 0.001), blood glucose ( = -0.40/ < 0.0001), triglycerides ( = -0.34/ < 0.0001), and TNF-alpha ( = -0.48/ < 0.0001), whereas a strongly positive correlation was found with IL-10 ( = 0.77/ < 0.0001). Multiple linear regression analysis revealed that behavior of IL-6 was mainly influenced by IL-10 (beta = 0.28/ = 1.95 × 10), TNF-alpha (beta = -0.67/ = 0.0017), and body fat percentage (beta = -5.95/ = 7.67 × 10) in women. In contrast, IL-10 (beta = 0.37/ = 1.34 × 10), TNF-alpha (beta = -0.85/ = 0.0005), and triglycerides (beta = 1.07/ = 0.0007) were major influencing factors of IL-6 in men. This study demonstrates that IL-6 is a marker of metabolic dysfunction that is differentially regulated in obese women and men. [Figure: see text].
关于白细胞介素 (IL)-6 在肥胖相关代谢功能障碍中的作用，相关信息有限。因此，我们研究了正常体重、超重和肥胖个体的血清 IL-6 水平，并探讨了 IL-6 与高血糖、胰岛素抵抗、血脂异常和全身炎症的关系。本研究共纳入 103 名女性和男性。测量了人体测量参数、血糖、胰岛素、总胆固醇和甘油三酯。采用酶联免疫吸附试验 (ELISA) 测定肿瘤坏死因子-α (TNF-α)、IL-10 和 IL-6 的血清水平。单因素方差分析 (ANOVA) 显示，与正常体重对照组相比，超重和肥胖个体的血清 IL-6 显著降低了 2.5 倍。血清 IL-6 与体重指数 (r = -0.39/ < 0.0001)、腰围 (r = -0.42/ < 0.001)、血糖 (r = -0.40/ < 0.0001)、甘油三酯 (r = -0.34/ < 0.0001) 和 TNF-α (r = -0.48/ < 0.0001) 呈显著负相关，而与 IL-10 呈强正相关 (r = 0.77/ < 0.0001)。多元线性回归分析显示，女性 IL-6 的行为主要受 IL-10 (β=0.28/=1.95 × 10)、TNF-α (β=-0.67/=0.0017) 和体脂百分比 (β=-5.95/=7.67 × 10) 的影响。相比之下，IL-10 (β=0.37/=1.34 × 10)、TNF-α (β=-0.85/=0.0005) 和甘油三酯 (β=1.07/=0.0007) 是男性 IL-6 的主要影响因素。本研究表明，IL-6 是代谢功能障碍的标志物，在肥胖女性和男性中的调节方式不同。[图：见正文]。
