酵母的无义抑制子会导致对渗透压敏感的生长。
Nonsense suppressors of yeast cause osmotic-sensitive growth.
作者信息
Singh A
出版信息
Proc Natl Acad Sci U S A. 1977 Jan;74(1):305-9. doi: 10.1073/pnas.74.1.305.
Abstract
Many nonsense suppressors of Saccharomyces cerevisiae cause growth inhibition on hypertonic media. Eight tyrosine-inserting UAA (ochre) suppressors, eight tyrosine-inserting UAG (amber) suppressors, a leucine-inserting UAG suppressor, and a serine-inserting recessive lethal UAG suppressor cause osmotic sensitivity, whereas a serine-inserting UAA suppressor does not cause sensitivity. Although the mechanism is not understood, the growth inhibition of specific suppressors on hypertonic media is correlated with their efficiencies of suppression. This heretofore unknown property of nonsense suppressors is useful for mitotic mapping, selecting tRNA mutants, selecting antisuppressors, and scoring nonsense suppressors.
摘要
酿酒酵母的许多无义抑制子会导致在高渗培养基上生长受抑制。八个插入酪氨酸的UAA（赭石型）抑制子、八个插入酪氨酸的UAG（琥珀型）抑制子、一个插入亮氨酸的UAG抑制子以及一个插入丝氨酸的隐性致死UAG抑制子会导致渗透敏感性，而一个插入丝氨酸的UAA抑制子不会导致敏感性。尽管其机制尚不清楚，但特定抑制子在高渗培养基上的生长抑制与其抑制效率相关。无义抑制子这种此前未知的特性可用于有丝分裂作图、筛选tRNA突变体、筛选反抑制子以及对无义抑制子进行评分。
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