Mahmoodi Seyed Reza, Xie Pengfei, Javanmard Mehdi
Annu Int Conf IEEE Eng Med Biol Soc. 2019 Jul;2019:1575-1578. doi: 10.1109/EMBC.2019.8857079.
We present a novel method for label-free detection of cytokines in serum after ten minutes of stabilization at nanomolar concentrations. Detection of proteins in blood using label-free impedance-based techniques is difficult due to high salt concentration of the matrix, which results in screening of the charge of the target proteins. The microwell array design provides enhanced electrochemical sensitivity by electric field focusing in the wells. We describe an on-chip multiwell plate sensing configuration where sensitivity benefits from the high salt concentration of the matrix and demonstrate robust performance through testing in rat serum.
我们提出了一种新方法，可在血清中细胞因子稳定十分钟后以纳摩尔浓度进行无标记检测。由于基质盐浓度高，使用基于无标记阻抗的技术检测血液中的蛋白质很困难，这会导致目标蛋白质电荷被屏蔽。微孔阵列设计通过孔内电场聚焦提高了电化学灵敏度。我们描述了一种芯片上的多孔板传感配置，其灵敏度得益于基质的高盐浓度，并通过在大鼠血清中的测试展示了强大的性能。
