INSERM U932, PSL University, Institut Curie, Paris, France.
INSERM U932, PSL University, Institut Curie, Paris, France.
Methods Enzymol. 2020;631:107-135. doi: 10.1016/bs.mie.2019.05.048. Epub 2019 Jul 25.
This chapter summarizes the recent findings regarding the development of gene-editing technologies applied to adoptive T cell immunotherapy for cancer. The emerging tools and strategies described below enable the generation of a tailored system, establishing T cells as an advanced biomaterial with redirected specificities that are both programmable and inducible and establish long-term antitumor potential. These reviewed techniques and strategies are implemented with an optimized protocol for the generation of CAR-T cells using lentiviral gene transfer.
本章总结了最近关于基因编辑技术在癌症过继性 T 细胞免疫治疗中的应用的研究结果。下面描述的新兴工具和策略使人们能够生成一个定制化系统，将 T 细胞作为一种具有可编程和诱导特异性的先进生物材料，并建立长期抗肿瘤潜力。这些经过审查的技术和策略是通过使用慢病毒基因转移来生成 CAR-T 细胞的优化方案来实施的。
