Wu Jian, Wang Shu-Tong, Zhang Zi-Jian, Zhou Qi, Peng Bao-Gang
Department of Hepatic Surgery, The First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong Province, PR China.
Department of General Surgery, The Seventh Affiliated Hospital of Sun Yat-sen University Shenzhen, Guangdong Province, PR China.
Int J Clin Exp Pathol. 2018 Oct 1;11(10):4908-4916. eCollection 2018.
CAMP responsive element binding protein 5 (CREB5) has been reported to be overexpressed in several types of human cancers and has crucial roles in regulating cell growth, proliferation, differentiation, and the cell cycle. However, the expression and function of CREB5 in hepatocellular carcinoma (HCC) remains unclear. The purpose of this study was to investigate the role of CREB5 in HCC, and its prognostic significance. We measured the expression of CREB5 in 91 specimens of paraffin-embedded HCC tissue by immunohistochemistry and performed a clinicopathological analysis. Gain of function and loss of function assays were used to evaluate the effect of CREB5 on cell proliferation . We found that up-regulation of CREB5 was associated with a poor prognosis, and CREB5 status was an independent prognostic factor. The overexpression of CREB5 increased the proliferation of SMMC-7721 cells, but the knockdown of CREB5 had the opposite effect. The results indicate that CREB5 may be useful when determining a treatment strategy for patients with HCC.
据报道，环磷腺苷效应元件结合蛋白5（CREB5）在多种人类癌症中过表达，在调节细胞生长、增殖、分化和细胞周期中起关键作用。然而，CREB5在肝细胞癌（HCC）中的表达和功能仍不清楚。本研究的目的是探讨CREB5在HCC中的作用及其预后意义。我们通过免疫组织化学检测了91例石蜡包埋的HCC组织标本中CREB5的表达，并进行了临床病理分析。采用功能获得和功能丧失试验来评估CREB5对细胞增殖的影响。我们发现CREB5的上调与预后不良相关，且CREB5状态是一个独立的预后因素。CREB5的过表达增加了SMMC-7721细胞的增殖，但敲低CREB5则产生相反的效果。结果表明，在确定HCC患者的治疗策略时，CREB5可能具有参考价值。
