Nishihira T, Kunori T, Tan M, Tsutsumi E, Kasai M
Tohoku J Exp Med. 1977 Jan;121(1):69-75. doi: 10.1620/tjem.121.69.
Anti-human B lymphocyte serum (AHBS) was obtained by absorbing rabbit anti-human tonsil serum (ATOS) or rabbit anti-human spleen serum (ASPS) with human red cells, liver, neutrophils, thymocytes and glutaraldehyde insolubilized human serum. Specificities of AHBS were checked by the cytotoxicity test, inhibition test of EAC-rosetting and inhibition of PWM responses. Raji cells of Burkitt's lymphoma originating from B-cells and acknowledged as non-immunoglobulin bearing on the cell surface were stained with AHBS according to the immunofluorescent technique. ATOS showed a higher cytotoxic titer against peripheral B-cells and inhibited EAC-rosetting stronger than ASPS did. On the other hand, macrophages in peripheral blood, Kupffer cells in the liver and certain cells of the thymus-medulla were stained with ASPA, but not with ATOS, even after intensively absorbing ASPS with thymocytes.
抗人B淋巴细胞血清（AHBS）是通过用人红细胞、肝脏、中性粒细胞、胸腺细胞和戊二醛固定的人血清吸收兔抗人扁桃体血清（ATOS）或兔抗人脾脏血清（ASPS）而获得的。通过细胞毒性试验、EAC玫瑰花结形成抑制试验和PWM反应抑制试验来检测AHBS的特异性。根据免疫荧光技术，用AHBS对源自B细胞且公认细胞表面不带有免疫球蛋白的伯基特淋巴瘤的Raji细胞进行染色。ATOS对外周B细胞显示出更高的细胞毒性滴度，并且比ASPS更能强烈抑制EAC玫瑰花结形成。另一方面，外周血中的巨噬细胞、肝脏中的枯否细胞以及胸腺髓质的某些细胞，即使在用胸腺细胞充分吸收ASPS后，也能用ASPA染色，但不能用ATOS染色。
