Nishihira T, Kunori T, Tan M, Tsutsumi E, Kasai M
Tohoku J Exp Med. 1977 Jan;121(1):63-8. doi: 10.1620/tjem.121.63.
A highly specific anti-human T lymphocyte serum (AHTS) was obtained by sequentially absorbing rabbit anti-human thymocyte serum (ATS) with human red cells, neutrophils, Burkitt's lymphoma cells (Daudi cells), liver, kidney and glutaraldehyde insolubilized human serum. The immunofluorescent staining revealed that the basement membrane of human renal glomeruli reacts with AHTS before being absorbed with kidney. Specificities of AHTS were studied by the cytotoxicity test, inhibition of PHA responses, inhibition of E-resulting and immunofluorescent staining.
通过用人红细胞、中性粒细胞、伯基特淋巴瘤细胞（Daudi细胞）、肝脏、肾脏以及戊二醛固定的人血清依次吸收兔抗人胸腺细胞血清（ATS），获得了一种高度特异性的抗人T淋巴细胞血清（AHTS）。免疫荧光染色显示，人肾小球基底膜在用肾脏吸收之前就与AHTS发生反应。通过细胞毒性试验、PHA反应抑制、E-花环形成抑制和免疫荧光染色研究了AHTS的特异性。
