Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
Virulence. 2020 Dec;11(1):104-112. doi: 10.1080/21505594.2020.1713649.
This study investigates the twitching ability of 28 clinical and five environmental strains of grown under iron-depleted condition through , phenotypic and proteomics approaches. Rapid Annotations using Subsystem Technology (RAST) analysis revealed the presence of 21 targets of type IV pilus shared across strains K279a, R551-3, D457 and JV3. The macroscopic twitching assay showed that only clinical isolates produced a zone of twitching with a mean of 22.00 mm under normal and 25.00 mm under iron-depleted conditions. (p = 0.002). Environmental isolates did not show any significant twitching activity in both conditions tested. Isobaric Tags for Relative and Absolute Quantification (ITRAQ) analysis showed altered expression of twitching motility protein PilT (99.08-fold change), flagellar biosynthesis protein FliC (20.14-fold change), and fimbrial protein (0.70-fold change) in response to iron-depleted condition. Most of the strains that have the ability to twitch under the normal condition, exhibit enhanced twitching during iron limitation.
本研究通过基因、表型和蛋白质组学方法，研究了在缺铁条件下生长的 28 株临床株和 5 株环境株的抽搐能力。通过快速注释子系统技术（RAST）分析发现，21 个 IV 型菌毛靶点存在于 K279a、R551-3、D457 和 JV3 等菌株中。宏观抽搐试验表明，只有临床分离株在正常条件下产生了 22.00mm 的抽搐区，在缺铁条件下产生了 25.00mm 的抽搐区。（p=0.002）。在两种测试条件下，环境分离株均无明显的抽搐活性。同量异位标记相对和绝对定量（ITRAQ）分析显示，在缺铁条件下，抽搐运动蛋白 PilT（99.08 倍变化）、鞭毛生物合成蛋白 FliC（20.14 倍变化）和菌毛蛋白（0.70 倍变化）的表达发生改变。大多数在正常条件下具有抽搐能力的菌株，在缺铁限制下表现出增强的抽搐能力。
