伊朗临床分离株中假定毒素-抗毒素系统的评估。
Evaluation of Putative Toxin-antitoxins Systems in Clinical in Iran.
机构信息
Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
出版信息
Infect Disord Drug Targets. 2021;21(1):38-42. doi: 10.2174/1871526520666200117113420.
BACKGROUND
Toxin-antitoxin systems (TAs) are two-component elements, which are extensive in the bacterial genome and have a regulatory role in many cellular activities including, growth arrest, survival, biofilm formation, and bacterial persistence.
OBJECTIVE
TAs have not well studied in Brucella spp.
METHODS
We evaluated the presence of different toxin-antitoxin systems, including relE- rhhlike, Fic- Phd, Cog- Rhh, and cogT- cogAT in 40 clinical Brucella melitensis isolates using PCRbased sequencing assay.
RESULTS
Our results showed the high presence of relE-rhh-like, Fic-Phd, Cog-rhh, and cogTcogAT s TAs genes in B. melitensis isolates that were 96.25%, 92.5%, 96.25%, and 95%, respectively.
CONCLUSION
A high presence of TAs genes in clinical B. melitensis isolates revealed that the TA system could be an antibacterial target in B. melitensis but more investigation is necessitated to elucidate the exact roles of these genes.
背景
毒素-抗毒素系统（TA）是由两个部分组成的元素，广泛存在于细菌基因组中，在许多细胞活动中具有调节作用，包括生长停滞、存活、生物膜形成和细菌持久性。
目的
布鲁氏菌属中 TA 的研究并不充分。
方法
我们使用基于 PCR 的测序检测评估了 40 株临床分离的布鲁氏菌 melitensis 中不同的毒素-抗毒素系统，包括 relE-rhhlike、Fic-Phd、Cog-rhh 和 cogT-cogAT。
结果
我们的结果显示，relE-rhh-like、Fic-Phd、Cog-rhh 和 cogTcogAT 等 TA 基因在布鲁氏菌 melitensis 分离株中的存在率很高，分别为 96.25%、92.5%、96.25%和 95%。
结论
临床分离的布鲁氏菌 melitensis 中 TA 基因的高存在表明 TA 系统可能是布鲁氏菌的一个抗菌靶标，但需要进一步研究来阐明这些基因的确切作用。
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