Belcher N G, Murdoch R D, Dalton N, House F R, Clark T J, Rees P J, Lee T H
Department of Medicine, Guy's Hospital, London, United Kingdom.
Am Rev Respir Dis. 1988 May;137(5):1026-32. doi: 10.1164/ajrccm/137.5.1026.
Serum neutrophil chemotactic activity (NCA) and plasma histamine concentrations were measured in 9 asthmatic subjects with exercise-induced asthma after inhalation challenge with ultrasonically nebulized 3.6% hypertonic saline, which was administered either in a dose-dependent manner (HSDR) or as a continuous single dose (HSC), and after cycle ergometer exercise. The mean decreases in FEV1 elicited by HSDR, HSC, and exercise were 26, 27, and 25%, respectively, and were not significantly different. There was an approximate 300% maximal increase in NCA detected after both HSC and exercise challenges. Gel filtration chromatography on columns of Ultragel ACA 34 indicated that the NCA released after HSC provocation and exercise were 600 to 700 kDa. There was an approximate 100% maximal increase in NCA after HSDR challenge, and this was significantly less (p = 0.016) than that after HSC and exercise. Exercise but not hypertonic challenge was associated with a basophilia and a significant increase in plasma histamine. There was a significant increase in plasma norepinephrine concentrations after exercise but not after HSC challenge in 7 asthmatics. Epinephrine concentrations did not change after exercise or HSC inhalation. NCA was measured in 5 subjects subjected to 2 HSC challenges that were separated by 60 min. There was an increase in NCA detected after both provocations. The increase after the second challenge was significantly greater (p = 0.27 x 10(-4)) than that observed after the initial provocation, despite a substantially reduced bronchoconstrictor response after the second challenge.
对9名运动诱发性哮喘的哮喘患者进行了研究，在超声雾化吸入3.6%高渗盐水后，分别以剂量依赖性方式（HSDR）或连续单次剂量（HSC）给药，以及在进行踏车测力计运动后，测量血清中性粒细胞趋化活性（NCA）和血浆组胺浓度。HSDR、HSC和运动引起的FEV1平均下降分别为26%、27%和25%，差异无统计学意义。在HSC和运动激发后，均检测到NCA最大增加约300%。在Ultragel ACA 34柱上进行凝胶过滤层析表明，HSC激发和运动后释放的NCA为600至700 kDa。HSDR激发后NCA最大增加约100%，显著低于（p = 0.016）HSC和运动后的增加。运动而非高渗激发与嗜碱性粒细胞增多和血浆组胺显著增加有关。7名哮喘患者运动后血浆去甲肾上腺素浓度显著增加，而HSC激发后未增加。运动或吸入HSC后肾上腺素浓度未改变。对5名受试者进行了两次间隔60分钟的HSC激发，并测量了NCA。两次激发后均检测到NCA增加。尽管第二次激发后的支气管收缩反应大幅降低，但第二次激发后的增加显著大于（p = 0.27 x 10(-4)）首次激发后的增加。
