• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在柑橘叶片圆盘上对 'Candidatus Liberibacter asiaticus' DNA 进行控制复制。

Controlled replication of 'Candidatus Liberibacter asiaticus' DNA in citrus leaf discs.

机构信息

Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USA.

Institute of Biological Chemistry, Washington State University, Pullman, WA, USA.

出版信息

Microb Biotechnol. 2020 May;13(3):747-759. doi: 10.1111/1751-7915.13531. Epub 2020 Jan 20.

DOI:10.1111/1751-7915.13531
PMID:31958876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7111093/
Abstract

'Candidatus Liberibacter asiaticus' is a fastidious bacterium and a putative agent of citrus greening disease (a.k.a., huanglongbing, HLB), a significant agricultural disease that affects citrus fruit quality and tree health. In citrus, 'Ca. L. asiaticus' is phloem limited. Lack of culture tools to study 'Ca. L. asiaticus' complicates analysis of this important organism. To improve understanding of 'Ca. L. asiaticus'-host interactions including parameters that affect 'Ca. L. asiaticus' replication, methods suitable for screening pathogen responses to physicochemical and nutritional variables are needed. We describe a leaf disc-based culture assay that allows highly selective measurement of changes in 'Ca. L. asiaticus' DNA within plant tissue incubated under specific physicochemical and nutritional conditions. qPCR analysis targeting the hypothetical gene CD16-00155 (strain A4) allowed selective quantification of 'Ca. L. asiaticus' DNA content within infected tissue. 'Ca. L. asiaticus' DNA replication was observed in response to glucose exclusively under microaerobic conditions, and the antibiotic amikacin further enhanced 'Ca. L. asiaticus' DNA replication. Metabolite profiling revealed a moderate impact of 'Ca. L. asiaticus' on the ability of leaf tissue to metabolize and respond to glucose.

摘要

“亚洲韧皮杆菌”是一种苛刻的细菌，也是柑橘黄龙病（又名黄龙病，HLB）的假定病原体，这是一种严重影响柑橘果实质量和树木健康的农业疾病。在柑橘中，“Ca. L. asiaticus”局限于韧皮部。缺乏用于研究“Ca. L. asiaticus”的培养工具，使得分析这个重要生物变得复杂。为了更好地了解“Ca. L. asiaticus”-宿主相互作用，包括影响“Ca. L. asiaticus”复制的参数，需要适合筛选病原体对物理化学和营养变量反应的方法。我们描述了一种基于叶圆盘的培养测定法，该方法允许在特定物理化学和营养条件下孵育的植物组织中高度选择性地测量“Ca. L. asiaticus”DNA 的变化。针对假设基因 CD16-00155（菌株 A4）的 qPCR 分析允许选择性地定量感染组织内的“Ca. L. asiaticus”DNA 含量。仅在微需氧条件下观察到“Ca. L. asiaticus”对葡萄糖的复制，抗生素阿米卡星进一步增强了“Ca. L. asiaticus”DNA 的复制。代谢物分析显示“Ca. L. asiaticus”对叶片组织代谢和响应葡萄糖的能力有适度影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/9c4a221a2f5d/MBT2-13-747-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/4905e3dac6d3/MBT2-13-747-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/3bcf1362af50/MBT2-13-747-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/e0ff1372b98a/MBT2-13-747-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/6cc7a482206e/MBT2-13-747-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/b5f016244546/MBT2-13-747-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/49936821ae4b/MBT2-13-747-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/9c4a221a2f5d/MBT2-13-747-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/4905e3dac6d3/MBT2-13-747-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/3bcf1362af50/MBT2-13-747-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/e0ff1372b98a/MBT2-13-747-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/6cc7a482206e/MBT2-13-747-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/b5f016244546/MBT2-13-747-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/49936821ae4b/MBT2-13-747-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7892/7111093/9c4a221a2f5d/MBT2-13-747-g007.jpg

相似文献

1
Controlled replication of 'Candidatus Liberibacter asiaticus' DNA in citrus leaf discs.在柑橘叶片圆盘上对 'Candidatus Liberibacter asiaticus' DNA 进行控制复制。
Microb Biotechnol. 2020 May;13(3):747-759. doi: 10.1111/1751-7915.13531. Epub 2020 Jan 20.
2
'Candidatus Liberibacter americanus', associated with citrus huanglongbing (greening disease) in São Paulo State, Brazil.“类美国韧皮杆菌”，与巴西圣保罗州的柑橘黄龙病（青枯病）有关。
Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):1857-1862. doi: 10.1099/ijs.0.63677-0.
3
Quantitative distribution of 'Candidatus Liberibacter asiaticus' in citrus plants with citrus huanglongbing.柑橘黄龙病病株中‘亚洲韧皮杆菌’的定量分布
Phytopathology. 2009 Feb;99(2):139-44. doi: 10.1094/PHYTO-99-2-0139.
4
Confirmation of the sequence of 'Candidatus Liberibacter asiaticus' and assessment of microbial diversity in Huanglongbing-infected citrus phloem using a metagenomic approach.利用宏基因组学方法确认‘亚洲韧皮杆菌’的序列并评估感染黄龙病的柑橘韧皮部中的微生物多样性。
Mol Plant Microbe Interact. 2009 Dec;22(12):1624-34. doi: 10.1094/MPMI-22-12-1624.
5
First Report of the Citrus Huanglongbing Associated Bacterium 'Candidatus Liberibacter asiaticus' from Sweet Orange, Mexican Lime, and Asian Citrus Psyllid in Belize.柑橘黄龙病相关细菌“亚洲韧皮杆菌”在伯利兹甜橙、墨西哥莱檬和亚洲柑橘木虱上的首次报道
Plant Dis. 2010 Jun;94(6):781. doi: 10.1094/PDIS-94-6-0781A.
6
One Target, Two Mechanisms: The Impact of 'Candidatus Liberibacter asiaticus' and Its Vector, Diaphorina citri, on Citrus Leaf Pigments.一个靶标，两种机制：“亚洲韧皮杆菌”及其载体柑橘木虱对柑橘叶片色素的影响。
Mol Plant Microbe Interact. 2017 Jul;30(7):543-556. doi: 10.1094/MPMI-02-17-0045-R. Epub 2017 May 8.
7
Graft transmission efficiencies and multiplication of 'Candidatus Liberibacter americanus' and 'ca. Liberibacter asiaticus' in citrus plants.“美洲韧皮杆菌”和“亚洲韧皮杆菌”在柑橘类植物中的嫁接传播效率及增殖情况
Phytopathology. 2009 Mar;99(3):301-6. doi: 10.1094/PHYTO-99-3-0301.
8
Heat treatment eliminates 'Candidatus Liberibacter asiaticus' from infected citrus trees under controlled conditions.热处理可在受控条件下从感染的柑橘树上消除‘亚洲韧皮杆菌’。
Phytopathology. 2013 Jan;103(1):15-22. doi: 10.1094/PHYTO-06-12-0138-R.
9
Genetic Diversity of the Indian Populations of 'Candidatus Liberibacter asiaticus' Based on the Tandem Repeat Variability in a Genomic Locus.基于基因组位点串联重复序列变异性的印度‘亚洲韧皮杆菌’群体的遗传多样性
Phytopathology. 2015 Aug;105(8):1043-9. doi: 10.1094/PHYTO-09-14-0253-R. Epub 2015 Jul 15.
10
Detection of citrus huanglongbing-associated 'Candidatus Liberibacter asiaticus' in citrus and Diaphorina citri in Pakistan, seasonal variability, and implications for disease management.巴基斯坦柑橘黄龙病相关的“亚洲韧皮杆菌”在柑橘和柑橘木虱中的检测、季节性变化及其对疾病管理的影响。
Phytopathology. 2014 Mar;104(3):257-68. doi: 10.1094/PHYTO-08-13-0224-R.

引用本文的文献

1
Rootstock and Crop Load Effects on 'Honeycrisp' Photosynthetic Performance and Carbohydrate Accumulation.砧木和作物负载对‘蜜脆’光合性能及碳水化合物积累的影响
Plants (Basel). 2023 Nov 30;12(23):4035. doi: 10.3390/plants12234035.
2
A synthetic 'essentialome' for axenic culturing of 'Candidatus Liberibacter asiaticus'.用于无菌培养“亚洲韧皮杆菌”的合成“必需组”。
BMC Res Notes. 2022 Apr 1;15(1):125. doi: 10.1186/s13104-022-05986-5.
3
Molecular signatures between citrus and Candidatus Liberibacter asiaticus.柑橘与亚洲韧皮杆菌之间的分子特征。

本文引用的文献

1
Co-cultivation of 'Candidatus Liberibacter asiaticus' with Actinobacteria from Citrus with Huanglongbing.“亚洲韧皮杆菌”与柑橘黄龙病相关放线菌的共培养
Plant Dis. 2008 Nov;92(11):1547-1550. doi: 10.1094/PDIS-92-11-1547.
2
Live Population Dynamics of 'Candidatus Liberibacter asiaticus', the Bacterial Agent Associated with Citrus Huanglongbing, in Citrus and Non-Citrus Hosts.与柑橘黄龙病相关的细菌病原体“亚洲韧皮杆菌”在柑橘和非柑橘寄主中的活体种群动态
Plant Dis. 2014 Jul;98(7):876-884. doi: 10.1094/PDIS-08-13-0886-RE.
3
Alterations of Liberibacter asiaticus-Associated Microbiota Decrease Survival of L. asiaticus in Assays.
PLoS Pathog. 2021 Dec 9;17(12):e1010071. doi: 10.1371/journal.ppat.1010071. eCollection 2021 Dec.
4
The Role of the Xylem in Oxytetracycline Translocation within Citrus Trees.木质部在柑橘树中土霉素转运中的作用
Antibiotics (Basel). 2020 Oct 13;9(10):691. doi: 10.3390/antibiotics9100691.
5
Revisiting the Complex Pathosystem of Huanglongbing: Deciphering the Role of Citrus Metabolites in Symptom Development.重新审视黄龙病的复杂病理系统：解读柑橘代谢产物在症状发展中的作用。
Metabolites. 2020 Oct 13;10(10):409. doi: 10.3390/metabo10100409.
6
Melatonin Is Involved in Citrus Response to the Pathogen Huanglongbing via Modulation of Phytohormonal Biosynthesis.褪黑素通过调节植物激素生物合成参与柑橘对黄龙病病原体的响应。
Plant Physiol. 2020 Dec;184(4):2216-2239. doi: 10.1104/pp.20.00393. Epub 2020 Aug 25.
亚洲韧皮杆菌相关微生物群的改变降低了其在试验中的存活率。
Front Microbiol. 2018 Dec 21;9:3089. doi: 10.3389/fmicb.2018.03089. eCollection 2018.
4
MetaboAnalyst 4.0: towards more transparent and integrative metabolomics analysis.MetaboAnalyst 4.0：迈向更透明、更综合的代谢组学分析。
Nucleic Acids Res. 2018 Jul 2;46(W1):W486-W494. doi: 10.1093/nar/gky310.
5
Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme in .解析在. 中敲除主要代谢酶对适应的遗传和代谢机制。
Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):222-227. doi: 10.1073/pnas.1716056115. Epub 2017 Dec 18.
6
Dynamics of Huanglongbing-associated Bacterium Candidatus Liberibacter asiaticus in Citrus aurantifolia Swingle (Mexican Lime).亚洲韧皮杆菌在墨西哥莱檬（Citrus aurantifolia Swingle）中与黄龙病相关的动态变化
Pak J Biol Sci. 2017;20(3):113-123. doi: 10.3923/pjbs.2017.113.123.
7
Wholly ! Reconstructed Metabolic Profile of the Quintessential Bacterial Parasite of Eukaryotic Cells.完整体现！真核细胞典型细菌寄生物的重建代谢特征。
mBio. 2017 Sep 26;8(5):e00859-17. doi: 10.1128/mBio.00859-17.
8
Concomitant Loss of the Glyoxalase System and Glycolysis Makes the Uncultured Pathogen "Candidatus Liberibacter asiaticus" an Energy Scavenger.伴随糖氧裂合酶系统和糖酵解的丧失，未培养病原体“亚洲韧皮杆菌”成为了一种能源掠夺者。
Appl Environ Microbiol. 2017 Nov 16;83(23). doi: 10.1128/AEM.01670-17. Print 2017 Dec 1.
9
Physicochemical and Nutritional Requirements for Axenic Replication Suggest Physiological Basis for Niche Restriction.无菌复制的物理化学和营养需求提示生态位限制的生理基础。
Front Cell Infect Microbiol. 2017 May 31;7:190. doi: 10.3389/fcimb.2017.00190. eCollection 2017.
10
One Target, Two Mechanisms: The Impact of 'Candidatus Liberibacter asiaticus' and Its Vector, Diaphorina citri, on Citrus Leaf Pigments.一个靶标，两种机制：“亚洲韧皮杆菌”及其载体柑橘木虱对柑橘叶片色素的影响。
Mol Plant Microbe Interact. 2017 Jul;30(7):543-556. doi: 10.1094/MPMI-02-17-0045-R. Epub 2017 May 8.