College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
Fuzhou University Zhicheng College, Fuzhou, 350002, People's Republic of China.
Arch Virol. 2020 Mar;165(3):771-774. doi: 10.1007/s00705-019-04521-5. Epub 2020 Jan 20.
Long-distance migratory insects carry microorganisms that can potentially play a crucial role in the life cycles of their hosts. Here, we used Illumina and Sanger sequencing to determine the complete genome sequence of a novel circular Rep-encoding single-stranded (ss) DNA virus from an important migratory pest, Agrotis ipsilon (Hufnagel). The full genome of this new virus is about 2, 242 nt in length and shares 55-75% genome-wide pairwise sequence identity with members of the family Genomoviridae but 91% nucleotide sequence identity with finch-associated genomovirus 3 isolate S30P_D, which is tentatively abbreviated "FaGmV-3". Viral infection rates in A. ipsilon from Yantai, Langfang and Xinxiang were 4.5% (n = 88), 11.8% (n = 85) and 0% (n = 35), respectively. Phylogenetic analysis based on the deduced amino acid sequence of Rep indicated that the Agrotis ipsilon-associated virus is closely related to members of the genus Gemykibivirus, and we propose it to be a new member of this genus. Hence, it is tentatively named "Agrotis ipsilon-associated genomovirus" (AiGmV).
长距离迁徙昆虫携带的微生物可能在其宿主的生命周期中发挥关键作用。在这里，我们使用 Illumina 和 Sanger 测序技术，从重要的迁徙害虫斜纹夜蛾（Agrotis ipsilon）中确定了一种新型环状 Rep 编码单链（ss）DNA 病毒的完整基因组序列。这种新病毒的全长约为 2242nt，与基因组病毒科的成员具有 55-75%的全基因组序列同一性，但与雀相关基因组病毒 3 分离株 S30P_D 的核苷酸序列同一性为 91%，后者暂被缩写为“FaGmV-3”。在烟台、廊坊和新乡的斜纹夜蛾中，病毒感染率分别为 4.5%（n=88）、11.8%（n=85）和 0%（n=35）。基于 Rep 推导的氨基酸序列进行的系统发育分析表明，与斜纹夜蛾相关的病毒与 Gemykibivirus 属的成员密切相关，我们提议将其作为该属的一个新成员。因此，它被暂命名为“斜纹夜蛾相关基因组病毒”（AiGmV）。
