Goularte Karina L, Voloski Flávia L S, Redú Josiara F M, Ferreira Carlos E R, Vieira Arnaldo D, Duval Eduarda H, Mondadori Rafael G, Lucia Thomaz
ReproPel, Universidade Federal de Pelotas, Pelotas-RS, Brazil.
Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brazil.
Reprod Domest Anim. 2020 Mar;55(3):318-324. doi: 10.1111/rda.13621. Epub 2020 Jan 21.
Many microorganisms from various sources may be present in ejaculates of bulls. This study identified and isolated bacteria from bull sperm samples in a commercial stud and evaluated their resistance to antibiotics. The number of colony-forming units was determined in semen samples collected at distinct steps during freezing and thawing. The minimum inhibitory concentration and the minimum bactericidal concentration were determined for four antibiotics commonly used in commercial studs. A total of 135 microorganisms from 25 genera were isolated. After a sensitivity test, all evaluated microorganisms (n = 55) were resistant to penicillin and most of them were resistant to tylosin and lincomycin (n = 54). Resistance to all tested antibiotics was observed in 22% of all isolates, whereas only 3.9% of the isolates were inhibited by the tested antibiotics at the concentrations recommended by the international legislation. As the isolated microorganisms presented high resistance to frequently used antibiotics, sensitivity tests should be periodically conducted in commercial bull semen studs to prevent the use of contaminated semen in artificial insemination.
公牛的精液中可能存在来自各种来源的多种微生物。本研究从一家商业种公牛站的公牛精子样本中鉴定并分离出细菌，并评估了它们对抗生素的耐药性。在冷冻和解冻过程的不同阶段收集的精液样本中测定了菌落形成单位的数量。测定了商业种公牛站常用的四种抗生素的最低抑菌浓度和最低杀菌浓度。共分离出25个属的135种微生物。经过敏感性测试，所有评估的微生物（n = 55）对青霉素耐药，其中大多数对泰乐菌素和林可霉素耐药（n = 54）。在所有分离株中，22%对所有测试抗生素耐药，而只有3.9%的分离株在国际法规推荐的浓度下被测试抗生素抑制。由于分离出的微生物对常用抗生素呈现出高度耐药性，商业公牛精液站应定期进行敏感性测试，以防止在人工授精中使用受污染的精液。
